Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Andreas Hofmann (Edited by), Samuel Clokie (Edited by)

9781107162273, Cambridge University Press

Hardback, published 19 April 2018

956 pages, 374 colour illus.
25.3 x 18.5 x 4.5 cm, 2.02 kg

Review from previous edition: 'I found this volume remarkably satisfying in that it answered all those 'why do you do it like this?' type questions. … This book has already received extensive reference value on my bookshelf.' Journal of Biological Education

Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, and bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.

Foreword Keith Wilson and John Walker
Preface Samuel Clokie and Andreas Hofmann
Tables and resources Andreas Hofmann
1. Life science studies Samuel Clokie and Andreas Hofmann
2. Basic principles Parisa Amani and Andreas Hofmann
3. Cell culture techniques Anwar Baydoun
4. Recombinant DNA techniques and molecular cloning Ralph Rapley
5. Preparative biochemistry Samuel Clokie
6. Electrophoresis Ralph Rapley
7. Immunochemical techniques Katja Fischer
8. Flow cytometry John Grainger and Joanne Konkel
9. Radioisotope techniques Robert Slater
10. Clinical biochemistry Gillian Rumsby
11. Microscopy Stephen Paddock
12. Centrifugation Kay Ohlendieck and Stephen Harding
13. Spectroscopic techniques Anne Simon and Andreas Hofmann
14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald
15. Mass spectrometric techniques Sonja Hess and James MacRae
16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio
17. Fundamentals of cheminformatics Paul Taylor
18. The Python programming language Timothy Stevens
19. Processing and visualisation of experimental data Jean-Baptiste Cazier
20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser
21. Fundamentals of proteomics Sonja Hess and Michael Weiss
22. Fundamentals of metabolomics James MacRae
23. Enzymes and receptors Megan Cross and Andreas Hofmann
24. Drug discovery and development David Camp.

Subject Areas: Biotechnology [TCB], Cellular biology [cytology PSF], Molecular biology [PSD], Biochemical immunology [PSBM], DNA & Genome [PSAK1], Biophysics [PHVN], Microscopy [PDND], Immunology [MJCM], Metabolism [MFGM], Medical laboratory testing & techniques [MBGL]