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What's Wrong With My Mouse?
Behavioral Phenotyping of Transgenic and Knockout Mice
Jacqueline N. Crawley (Author)
9780471471929, Wiley
Hardback, published 17 April 2007
544 pages
25.4 x 18 x 3.3 cm, 1.089 kg
First Edition Reviews: "The book successfully brings together the field of behavioral neuroscience and the rewards and insights that can be gained using this approach. I would recommend that every behavioral scientist has at least two copies, one for their own use and one that will be on permanent load to their students, post-doctoral students and colleagues in molecular biology." (Trends in Pharmaceutical Sciences) "the overall impression is of a helpful guide taking the reader step by step through the complexities of animal behavioral research." (Nature Neuroscience) "succeeds as a useful introduction to the realm of behavioral phenotyping for those interested in creating or using the large and increasing number of promising targeted mutant mice." (Trends in Neurosciences)
Dr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse? Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions. The final chapter, "The Next Generation," examines new and emerging technologies. Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.
Preface ix Preface to the First Edition xi Acknowledgments xiii Chapter 1 Designer Mice 1 Chapter 2 Of Unicorns and Chimeras 10 Chapter 3 General Health 42 Chapter 4 Motor Functions 62 Chapter 5 Sensory Abilities 86 Chapter 6 Learning and Memory 110 Chapter 7 Feeding and Drinking 164 Chapter 8 Reproductive Behaviors 186 Chapter 9 Social Behaviors 206 Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226 Chapter 11 Reward 266 Chapter 12 Neurodevelopment and Neurodegeneration 290 Chapter 13 Putting It All Together 322 Chapter 14 The Next Generation 344 References 383 Index 503
Scope and sourcebooks
How to generate a line of transgenic or knockout mouse for behavioural phenotyping
Give your mouse a physical
Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures
Olfaction, vision, hearing, taste, touch, nociception
Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention
Daily consumption, restricted access, choice tests, microstructural analysis
Sexual and parental behaviors
Social interaction, nesting, grooming, juvenile play, aggression
Mouse models of fear, anxiety, depression, schizophrenia
Self-administration of addictive drugs, conditioned place preference
Assaying behaviors in infant, juvenile, and aged mice
Choice of tests, order of testing, number of mice, equipment, housing and testing environment
Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues
Subject Areas: Biology, life sciences [PS]
