Visualizing RNA Dynamics in the Cell

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field, with this volume focusing on RNA dynamics in the cell

Grigory S Filonov (Volume editor), Samie Jaffrey (Volume editor)

9780128022924

Hardback, published 27 May 2016

384 pages
22.9 x 15.1 x 2.6 cm, 0.81 kg

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience

Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe.

1. RNA Imaging with Multiplexed Error Robust Fluorescence in Situ Hybridization
J.R. Moffitt and X. Zhuang
2. Imaging Single mRNA Dynamics in Live Neurons and Brains
H.C. Moon and H.Y. Park
3. Monitoring of Rna Dynamics in Living Cells Using Pum-Hd and Fluorescent Protein Reconstitution Technique
H. Yoshimura and T. Ozawa
4. Applications of Hairpin DNA Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells
S.R. Jackson, A.C. Wong, A.R. Travis, I.E. Catrina, D.P. Bratu, D.W. Wright and A. Jayagopal
5. In Vivo RNA Visualization in Plants Using MS2 Tagging
E.J. Peña and M. Heinlein
6. Trick: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals
J.M. Halstead, J.H. Wilbertz, F. Wippich, T. Lionnet, A. Ephrussi and  J.A. Chao
7. Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory
A. Coulon and D.R. Larson
8. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters
J. Ray, I. Shin, M. Ilgu, L. Bendickson, V. Gupta, G.A. Kraus and M. Nilsen-Hamilton
9. A Method for Expressing and Imaging Abundant, Stable, Circular Rnas in Vivo Using Trna Splicing
C.A. Schmidt, J.J. Noto, G.S. Filonov and A.G. Matera
10. RNA-ID, A Powerful Tool for Identifying and Characterizing Regulatory Sequences
C.E. Brule, K.M. Dean and E.J. Grayhack
11. Fluorescent Protein-Based Quantification of Alternative Splicing of a Target Cassette Exon in Mammalian Cells
N.G. Gurskaya, D.B. Staroverov and K.A. Lukyanov
12. Iras: High-Throughput Identification of Novel Alternative Splicing Regulators
S. Zheng
13. Analysis of Nonsense-Mediated mRNA Decay at the Single Cell Level using Two Fluorescent Proteins
N.G. Gurskaya, A.P. Pereverzev, D.B. Staroverov, N.M. Markina and K.A. Lukyanov
14. Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells
J.L. Litke, M. You and S.R. Jaffrey

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Microscopy [PDND]