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Understanding PCR
A Practical Bench-Top Guide
A user friendly book that features step-by-step basic protocols, helpful resource recommendations, and basic troubleshooting tips, this approachable and practical guide explains the process for setting up and analyzing one's first PCR reaction
Sarah Maddocks (Author), Rowena Jenkins (Author)
9780128026830, Elsevier Science
Paperback, published 26 October 2016
98 pages
22.9 x 15.1 x 0.8 cm, 0.18 kg
Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.
Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers Chapter 3. Analyzing Your Template DNA and/or PCR Product Chapter 4. Quantitative PCR: Things to Consider Chapter 5. Carrying Out Q-PCR Chapter 6. Using PCR for Cloning and Protein Expression Chapter 7. Polymerase Chain Reaction for Knocking Out Genes
Subject Areas: Molecular biology [PSD]
