1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish – Wenbiao Chen

2. Advances in Tol2 Transposon Mutagenesis – Koichi Kawakami

3. OPEN Zinc Finger Nucleases – Keith Joung and Randy Peterson

4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish – Adam Amsterdam, Nancy Hopkins, and Shawn Burgess

5. Genetic Screens for Maternal-Effects Mutations – Francisco Pelegri and Mary Mullins

6. High-Throughput Target-Selected Gene Inactivation in Zebrafish – Derek Stemple

7. Suppressor Screens in Haploids – Xiaoying Bai

8. Transposons – Stephen Ekker

9. Caged MOs – James Chen

Part II: Transgenesis

1. Advanced Transgenesis with Cre Recombinase – Christian Mosimann

2. Transgenesis with phiC31 Recombinase – James Lister

3. Nuclear Transfer – Jose Cibelli and Dr. Siripattarapravat

Part III: The Zebrafish Genome and Mapping Technologies

1. The Zebrafish Genome Project: Sequence Analysis and Annotation– Derek Stemple

2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish – Charles Lee and Amanda Smith

3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome – John Postlethwait, Julian Catchen

4. The Zon Laboratory Guide to Positional Cloning in Zebrafish –Yi Zhou and Leonard Zon

Part IV: Informatics and Comparative Genomics

1. Data Warehouse ZFIN – Monte Westerfield and Judy Sprague

2. Epigenetics: Methylation – Brad Cairns

3. Chromatin Structure – Leonard Zon and Yi Zhou

4. ChipSeq – Brad Cairns

5. Micro RNAs – Graham Lieshke and Luke Pase

6. Microarrays – Yi Zhou

7. Sequence-Based Transcriptional Profiling – Shawn Burgess

8. Epigenetic Regulation of Zebrafish Development – Elke Ober and co-author

Part V: Infrastructure

1. Zebrafish Facilities for Small and Large Laboratories – Christian Lawrence

2. Genetic Backgrounds, Standard Lines, and their Husbandry – ZIRC, Zoltan