The Immunological Synapse - Part B

Edited by one of the best scientists in the field, this series presents the latest updates in the field of cell biology

Clément Thomas (Volume editor), Lorenzo Galluzzi (Volume editor)

9780443191633, Elsevier Science

Hardback, published 11 July 2023

222 pages
22.9 x 15.2 x 2.4 cm, 0.45 kg

The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay.

Contributors in this volume include:
Torsten Tonn
Jiri Eitler
Rose Thierry
Clément Thomas
Jacques Zimmer
Carsten Watzl
Pedro Proda
Arnika Kathleen Wagner
Alicia Copik
Claudio Giraudo
Loic Dupre
Felix Borgmann
Chenguang Xu
Wanli Liu

Subject Areas: Molecular biology [PSD], Developmental biology [PSC]