The Immunological Synapse Part A

Edited by one of the best scientists in the field, this series presents the latest updates in the field of cell biology

Clément Thomas (Volume editor), Lorenzo Galluzzi (Volume editor)

9780323901543, Elsevier Science

Hardback, published 11 January 2023

206 pages
23.4 x 19 x 2 cm, 0.59 kg

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more.

1. Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators
   Marie Juzans, Celine Cuche, Vincenzo Di Bartolo, and Andres Alcover
2. Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse
   Sara Fernandez-Hermira, Irene Sanz-Fernandez, Marta Botas, Victor Calvo, and Manuel Izquierdo
3. P815-based redirected degranulation assay to study human NK cell effector functions
   Iñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego
4. Cytotoxic and chemotactic dynamics of NK cells quantified by live-cell imaging
   Yanting Zhu and Jue Shi
5. Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay
   Ondrej Cerny
6. Assessment of membrane lipid state at the natural killer cell immunological synapse
   Yu Li and Jordan S. Orange
7. Study of the effects of NK-tumor cell interaction by proteomic analysis and imaging
   Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi
8. Protocol for the murine antibody-dependent cellular phagocytosis assay
   Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto
9. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
   Melanie Grusdat, Catherine Dostert, and Dirk Brenner
10. An in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissue
    Nicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte Wieten
11. Standardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer
    Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu
12. Potency monitoring of CAR T cells
    Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC]