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Technology Platforms for 3D Cell Culture
A User's Guide

Stefan Przyborski (Edited by)

9781118851500, Wiley

Hardback, published 21 April 2017

424 pages
25.2 x 17.3 x 2.5 cm, 0.998 kg

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.

  • Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture
  • Features contributions from leading developers and researchers active in 3D cell technology
  • Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied
  • Includes information on resources and technical support to help initiate the use of 3D culture methods

List of contributors, vii

Preface, xi

List of abbreviations, xiv

1 An introduction to the third dimension for routine cell culture, 1
Antonio Romo]Morales and Stefan Przyborski

Part I: Aggregate]based technologies

2 Gravity]enforced microtissue engineering, 23
Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm

3 Physiologically relevant spheroid models for three]dimensionalcell culture, 50
Nicole A. Slawny and MaryAnn Labant

4 NanoCulture Plate: A scaffold]based high]throughput three]dimensional cell culture system suitable for live imaging and co]culture, 74
Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman

5 Micro]moulded non]adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish®, 97
Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan

6 Organotypic microtissues on an air]liquid interface, 123
Lars E. Sundstrom, Igor Charvet and Luc Stoppini

Part II: Hydrogels

7 Materials and assay systems used for three]dimensional cell culture, 145
Suparna Sanyal and Marshall Kosovsky

8 HyStem®, a customisable hyaluronan]based hydrogel matrix for 3D cell culture, 173
T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach]Carson and D. D. Carson

9 3]D Life biomimetic hydrogels: A modular system for cell environment design, 197
Brigitte M. Angres and Helmut Wurst

Part III: Scaffolds

10 Alvetex®, a highly porous polystyrene scaffold for routine three]dimensional cell culture, 225
Antonio Romo]Morales, Eleanor Knight and Stefan Przyborski

11 CelluSponge™ and Go Matrix as innovative three]dimensional cell culture platforms, 250
Bramasta Nugraha

12 Mimetix® electrospun scaffold: An easy]to]use tool for 3D cell culture in drug discovery and regenerative medicine, 284
Robert J. McKean and Elena Heister

Part IV: 3D bioreactor technologies

13 Quasi Vivo® bioreactor technology, 305
J. Malcolm Wilkinson

14 Three]dimensional cell]based assays in hollow fibre bioreactors, 327
John J. S. Cadwell and William G. Whitford

15 Three]dimensional engineered tissues for high]throughput compound screening: Mechanical properties of skin and ageing, 351
Michael Conway, Ayla Annac and Tetsuro Wakatsuki

16 Three]dimensional cell culture in the Rotary Cell Culture System™, 370
Stephen S. Navran

Glossary, 386

Index, 393

Subject Areas: Other branches of medicine [MM]
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