Techniques in Molecular Diagnostics

Kandarpa Kumar Saikia (Author)

9789358870268

Paperback / softback, published 25 August 2016

142 pages
25 x 15 x 1.5 cm, 0.4 kg

The text offers a comprehensive overview of molecular biology and diagnostics, ranging from fundamental concepts to advanced diagnostic assays that can be performed in a laboratory setting. It provides detailed information on establishing and operating a diagnostic laboratory, including laboratory mathematics, which is useful for preparing common laboratory solutions. Additionally, the book offers basic protocols for extracting nucleic acids from various sources, which can be applied to isolate high-quality DNA and RNA from human blood and tissues.

The book is also replete with various molecular diagnostic tools that are user-friendly and can be utilized in the diagnosis of different cancers. It provides examples of biomarker detection protocols for specific cancer types using molecular diagnostic tools, which can be easily replicated and are highly sensitive, cost-effective, and quick, resulting in faster and more accurate diagnoses. Finally, it discusses the quality assurance and quality control strategies of a molecular diagnostic laboratory, including the design of quality manuals for such laboratories, to ensure the highest standards of accuracy and reliability.

• 1. Introduction to Molecular Diagnostics by Partha Pratim Sarma
• 2. General Calculation for Making Solutions in Molecular Diagnostic Laboratory by Pranjal Kr. Baruah
• 3. Basic Requirements for Setting-up a Molecular Diagnostic Laboratory by Kandarpa Kr. Saikia
• 4. Nucleic Acid Extraction Protocols by Partha Pratim Sarma and Kandarpa Kr. Saikia
• 5. Protocol to Perform Agarose Gel Electrophoresis by Sukanta Nath and Partha Pratim Sarma
• 6. Protocol for Qualitative PCR Using Genomic DNA or cdna as Template by Kandarpa Kr. Saikia and Sukanta Nath
• 7. DNA Sequence Analysis by Kandarpa Kr. Saikia and Vedant Vikrom Borah
• 8. Protocol for Synthesis of cDNA from Total RNA by Sukanta Nath and Partha Pratim Sarma
• 9. Protocol to Perform RT-PCR (Reverse Transcriptase PCR for Gene Expression Studies) by Kandarpa Kr. Saikia
• 10. Protocol to Perform Quantitative Real Time PCR (qpcr) by Partha Pratim Sarma and Sukanta Nath
• 11. Protocol to Perform FISH by Manoj Kr. Panigrahi
• 12. Examples of Biomarker Detection Protocols Using Molecular Diagnostic Tools -Acute Myeloid Leukaemia, Glioma and Chronic Myeloid Leukaemia Molecular Markers by Kandarpa Kr. Saikia, Partha Pratim Sarma, Sukanta Nath and Dushyant Kumar
• 13. Quality Assurance and Quality Control in Molecular Diagnostics by Rupjyoti Kalita and Kandarpa Kr. Saikia