• Synthetic Biology, Part A; Methods for Part/Device Characterization and Chassis Engineering (Hardback) 9780123850751
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Synthetic Biology, Part A
Methods for Part/Device Characterization and Chassis Engineering

Synthetic biology encompasses several different approaches, methodologies and disciplines, and many different definitions exist. This volume of Methods in Enzymology has been split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA sssembly and manipulation.

Chris Voigt (Edited by)

9780123850751, Elsevier Science

Hardback, published 8 July 2011

714 pages
22.9 x 15.1 x 3.7 cm, 1.27 kg

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.

Section I. Measuring and Engineering Central Dogma Processes

  1. Sequence-specificity and Energy Landscapes of DNA-binding Molecules

    2. Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari

  2. Promoter Reliability in Modular Transcriptional Networks

    3. Rajat Anand, Navneet Rai and Mukund Thattai

  3. The Analysis of ChIP-seq Data

    4. Wenxiu Ma and Wing Hung Wong

  4. Using DNA Microarrays to Assay Part Function

    5. Virgil A. Rhodius and Carol A. Gross

  5. Orthogonal gene expression in Escherichia coli

    6. Wenlin An and Jason Chin

  6. Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation

    7. Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos

    Section II. Device and System Design, Optimization, and Debugging

  7. Design and Connection of Robust Genetic Circuits

    8. Ron Weiss

  8. Engineering RNAi circuits

    9. Yaakov Benenson

  9. From SELEX to cell: Dual selections for synthetic riboswitches

    10. Joy Sinha, Shana Topp, and Justin P. Gallivan

  10. Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells

    11. Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You

  11. De novo design and construction of an inducible gene expression system in mammalian cells

    12. Maria Karlsson, Wilfried Weber and Martin Fussenegger

  12. BioBuilding: Using banana-scented bacteria to teach synthetic biology

    13. James Dixon and Natalie Kuldell

    Section III. Device Measurement, Optimization, and Debugging

  13. Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics

    14. Gürol Süel

  14. Microfluidics for synthetic biology: From design to execution

    15. M. S. Ferry, I. A. Razinkov, J. Hasty

  15. Plate-based assays for light-regulated gene expression systems

    16. Jeffrey J. Tabor

  16. Spatiotemporal control of small GTPases with light using the LOV domain

    17. Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn

  17. Light Control of Plasma Membrane Recruitment using the Phy—PIF system

    18. Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner

  18. Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes

    19. Ed Boyden

    Section IV. Devices for Metabolic Engineering

  19. Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding

    20. John E. Dueber and Weston R. Whitaker

  20. A Synthetic Iterative Pathway for ketoacid elongation

    21. C. R. Shen, J. C. Liao

    Section V. Expanding Chasses

  21. Synthetic Biology in Streptomyces bacteria

    22. Marnix Medema, Rainer Breitling and Eriko Takano

  22. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio

    23. Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra

  23. Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons

    24. Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty

  24. Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions

    25. Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter Lindblad

  25. Developing a Synthetic Signal Transduction System in Plants

    26. Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. Medford

  26. Lentiviral Vectors to Study Stochastic Noise in Gene Expression

Kate Franz, Abhyudai Singh, and Leor S. Weinberger

Subject Areas: Biotechnology [TCB], Cellular biology [cytology PSF], Molecular biology [PSD], Genetics [non-medical PSAK]
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