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Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants

As the latest volume in the Methods in Enzymology series, this edition continues the legacy of this premier serial with quality chapters authored by experts in the field who discuss synthetic biology and metabolic engineering in plants and microbes

Sarah E O'Connor (Volume editor)

9780128045398

Hardback, published 28 July 2016

446 pages
22.9 x 15.1 x 2.8 cm, 0.88 kg

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience

Synthetic Biology and Metabolic Engineering in Plants and Microbes, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

This volume covers research methods, synthetic biology, and metabolic engineering in plants and microbes, and includes sections on such topics as the usage of integrases in microbial engineering, biosynthesis, and engineering of tryptophan derived metabolites, regulation and discovery of fungal natural products, and elucidation and localization of plant pathways.

1. Comparative Biochemistry and in Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic Diversity
Pengxiang Fan, Gaurav D. Moghe and Robert L. Last
2. De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural Products
Rongchun Han, Amit Rai, Michimi Nakamura, Hideyuki Suzuki, Hiroki Takahashi, Mami Yamazaki and Kazuki Saito
3. Genomics Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in Sandalwood Oil Biosynthesis
Jose M. Celedon  and Jörg Bohlmann
4. A Workflow for Studying Specialized Metabolism in Non-Model Eukaryotic Organisms
Michael P. Torrens-Spence, Timothy R. Fallon and  Jing-Ke Weng
5. Gene Discovery for Synthetic Biology: Exploring the Novel Natural Product Biosynthetic Capacity of Eukaryotic Microalgae
Ellis C. O’Neill, Gerhard Saalbach and Robert A. Field
6. Cis-Prenyltransferase and Polymer Analysis from a Natural Rubber Perspective
Moonhyuk Kwon, Eun-Joo Gina Kwon and Dae-Kyun Ro
7. Generation and Functional Evaluation of Designer Monoterpene Synthases
Narayanan Srividya, Iris Lange and Bernd Markus Lange
8. Prequels to Synthetic Biology: From Candidate Gene Identification and Validation to Enzyme Subcellular Localization in Plant and Yeast Cells
Emilien Foureau, Inês Carqueijeiro, Thomas Dugé de Bernonville, Céline Melin, Florent Lafontaine, Sébastien Besseau, Arnaud Lanoue, Nicolas Papon, Audrey Oudin, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre, Nathalie Giglioli-Guivarc’h and Vincent Courdavault
9. Functional Expression and Characterization of Plant ABC Transporters in Xenopus Laevis Oocytes for Transport Engineering Purposes
Deyang Xu, Dorottya Veres, Zeinu Mussa Belew, Carl Erik Olsen, Hussam Hassan Nour-Eldin and Barbara Ann Halkier
10. Quantifying the Metabolites of the Methylerythritol 4-Phosphate (Mep) Pathway in Plants and Bacteria by Liquid Chromatography – Triple Quadrupole Mass Spectrometry
Diego González-Cabanelas, Almuth Hammerbacher, Bettina Raguschke, Jonathan Gershenzon and Louwrance P. Wright
11. Establishing the Architecture of Plant Gene Regulatory Networks
Fan Yang, Wilberforce Zachary Ouma, Wei Li, Andrea I. Doseff, and Erich Grotewold
12. Engineering of Tomato Glandular Trichomes for the Production of Specialized Metabolites
Ruy W.J. Kortbeek, Jiesen Xu, Aldana Ramirez, Eleni Spyropoulou, Paul Diergaarden, Ilona Otten-Bruggeman, Michiel de Both, Raimund Nagel, Axel Schmidt, Robert C. Schuurink and Petra M. Bleeker
13. Tomato Fruits – a Platform for Metabolic Engineering of Terpenes
Michael Gutensohn, and Natalia Dudareva
14. Libraries of Synthetic Tale-Activated Promoters: Methods and Application
Tom Schreiber and Alain Tissier

Subject Areas: Biotechnology [TCB], Botany & plant sciences [PST], Microbiology [non-medical PSG], Cellular biology [cytology PSF]

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