Structural Genomics, Part A

This volume presents step-by-step techniques for researchers that will predict the structure and potential function of proteins by identifying its coding sequence

Andrzej Joachimiak (Edited by)

9780123744364, Elsevier Science

Hardback, published 18 December 2008

160 pages
22.9 x 15.1 x 1.8 cm, 0.44 kg

Structural genomics is the systematic determination of 3-dimensional structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome.

Introduction
Analysis of genomes for structural genomics and the limitations of sequence analysis
Structure determination pipeline for structural genomics
Gene cloning and protein expression
Gene cloning and expression of membrane proteins
High-throughput protein purification for x-ray crystallography and NMR
High-throughput protein crystallization
Structure determination using synchrotron radiation
Application of NMR to structural genomics
Development of key high-throughput technologies: High-throughput functional screening
Development of key high-throughput technologies: Structure prediction and homology modeling
Functional inferences from structure
Structural genomics programs (SARS case)
Structural genomics programs (E.coli)
Structural genomics as a structural foundation for drug discovery
Structural genomics of eukaryote
Structural genomics of complexes
Dissemination structural genomics data to biology community

Subject Areas: Proteins [PSBC], DNA & Genome [PSAK1]