Stem Cell Laboratory Techniques
A Guide for Researchers and Students

Introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers

Suresh Kumar Subbiah (Edited by), Akon Higuchi (Edited by), Pooi Ling Mok (Edited by)

9780128237298, Elsevier Science

Paperback, published 31 May 2023

240 pages, 70 illustrations (20 in full color)
27.6 x 21.6 x 1.6 cm, 0.45 kg

Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study.

1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and Application of Stem Cell
2. Extraction of Stem Cells from Animal Models
3. Harvest and Culture of Stem Cells
4. Establishment of Amniotic fluid Stem Cell
5. Stem Cell Characterization
6. Good Recording and Quality Control
7. Differentiation Techniques for Confirmation Studies
8. Transcriptomic Analysis
9. Proliferation Study and Analysis
10. Single Cell Isolation Methods and their Impact on Stem Cell Study
11. Gene Editing Techniques for Stem Cell
12. Preparation of Cells for in vitro study
13. Stem Cell Lab Design and Equipment
14. Human and Animal Research Ethics
15. Organoids

Subject Areas: Life sciences: general issues [PSA]