Quantitative Biomedical Optics
Theory, Methods, and Applications

Based on physical science principles, Quantitative Biomedical Optics covers theory, instrumentation, methods and applications, with practical exercises and problem sets.

Irving J. Bigio (Author), Sergio Fantini (Author)

9780521876568, Cambridge University Press

Hardback, published 7 January 2016

698 pages, 212 b/w illus. 8 tables
25.3 x 19.8 x 3.6 cm, 1.6 kg

'Educators in Biomedical Optics and Biophotonics have been long awaiting a comprehensive text to accompany their teaching in this rapidly growing field and this is as good as it gets. Educators may have their own bias, but this text provides a balanced approach giving due weight to the topics within the field and covering them comprehensively for most undergraduate and graduate courses in the field … There is sufficient depth, detail and reference material for very specialized researchers to use this as their 'bible' and it should be on the desk of every serious educator and researcher in biomedical optics.' Martin Leahy, National University of Ireland, Galway

This is the textbook and reference resource that instructors, students, and researchers in biomedical optics have been waiting for. Comprehensive and up to date, it covers a broad range of areas in biomedical optics, from light interactions at the single-photon and single-biomolecule levels, to the diffusion regime of light propagation in tissue. Subjects covered include spectroscopic techniques (fluorescence, Raman, infrared, near-infrared, and elastic scattering), imaging techniques (diffuse optical tomography, photoacoustic imaging, several forms of modern microscopy, and optical coherence tomography), and laser-tissue interactions, including optical tweezers. Topics are developed from the fundamental principles of physical science, with intuitive explanations, while rigorous mathematical formalisms of theoretical treatments are also provided. For each technique, descriptions of relevant instrumentation and examples of biomedical applications are outlined, and each chapter benefits from references and suggested resources for further reading, and exercise problems with answers to selected problems.

1. Nomenclature
2. Overview of tissue optical properties
3. Introduction to biomedical statistics for diagnostic applications
4. General concepts of tissue spectroscopy and instrumentation
5. Autofluorescence spectroscopy and reporter fluorescence
6. Raman and infrared spectroscopy of vibrational modes
7. Elastic and quasi-elastic scattering from cells and small structures
8. Diffuse reflectance spectroscopy at small source-detector separations
9. Transport theory and the diffusion equation
10. Continuous-wave methods for tissue spectroscopy
11. Time-domain methods for tissue spectroscopy in the diffusion regime
12. Frequency-domain methods for tissue spectroscopy in the diffusion regime
13. Instrumentation and experimental methods for diffuse tissue spectroscopy
14. Diffuse optical imaging and tomography
15. In vivo applications of diffuse optical spectroscopy and imaging
16. Combining light and ultrasound: acousto-optics and opto-acoustics
17. Modern optical microscopy for biomedical applications
18. Optical coherence tomography
19. Optical tweezers and laser-tissue interactions.

Subject Areas: Electrical engineering [THR], Biotechnology [TCB], Biochemical engineering [TC], Life sciences: general issues [PSA], Biophysics [PHVN]