Purification and Characterization of Secondary Metabolites
A Laboratory Manual for Analytical and Structural Biochemistry

A practical, comprehensive laboratory manual containing modular exercises with a focus on encouraging critical thinking and analysis

Thomas E. Crowley (Author)

9780128139424, Elsevier Science

Paperback, published 13 August 2019

214 pages
22.9 x 15.1 x 1.5 cm, 0.32 kg

Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data.

1. The Structure and Function of Secondary Metabolites that are Secreted by Bacteria2. The Reagents, Supplies and Equipment that are Necessary to Grow Cultures of Bacteria in the Laboratory and to Purify Secreted Metabolites3. Overview of the Methods for Purification of Metabolites that are Secreted by Bacteria4. Absorption Spectrophotometry: Ultraviolet-visible and Infrared5. High-performance Liquid Chromatography6. Mass Spectrometry7. X-ray Crystallography8. Nuclear Magnetic Resonance Spectroscopy9. Exercises in Purifying and Characterizing a Quorum-sensing Signal10. Exercises in Purifying and Characterizing Iron-chelating Molecules11. Exercises in Purifying and Characterizing a Chloroplast-targeting Phytotoxin12. Designing your Own Experiments

Subject Areas: Biochemistry [PSB], Biophysics [PHVN]