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Protocols in Biochemistry and Clinical Biochemistry
Offers applied, accessible instruction in research and clinical biochemistry protocols across a range of study types and diagnostics
Shweta Pandey (Author), Shyamal K Goswami (Author), Buddhi Prakash Jain (Author)
9780128220078, Elsevier Science
Paperback, published 16 November 2020
138 pages
23.4 x 19 x 1 cm, 0.29 kg
Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction to fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification, protein, lipid, carbohydrate, and enzyme testing, and clinical testing of vitamins, glucose and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labeled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods and troubleshooting.
Unit I: Buffer, solution and pH1. Preparation of normal and molar solution2. Buffer preparation3. Determination of pKa, pH Unit II: Nucleic Acids1. Isolation and Purification of DNA2. Isolation and Purification of RNA3. Purification of Nucleic Acid4. Quantitative estimation of Nucleic acid5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids1. Qualitative test for carbohydrate (Benedict, Molisch etc)2. Qualitative test for Lipids3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins1. Isolation of Protein2. Qualitative test of Protein (Biuret test, Xanthoproteic test, Ninhydrin test etc)3. Estimation of Protein (Bradford, Lowry etc)4. Paper Chromatography for separation and identification of amino acid5. Ion exchange chromatography for separation of amino acid6. Protein purification7. SDS PAGE analysis of proteins8. Western blotting for protein analysis9. Isoelectric pH determination Unit IV: Enzymes1. Enzyme kinetics2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity3. Enzymatic activity of Trypsin and Lipase4. Enzyme purification5. Determination of Km, Vmax and Ki Unit V: Clinical test1. Acid and Alkaline phosphatase activity2. SGPT and SGOT test3. Estimation of Urea, Uric acid and Creatinine4. Estimation of Glucose5. Estimation of Cholesterol6. Estimation of Vitamins7. Glucose tolerance test8. Estimation of Ca2+ and T4 in serum9. Pregnancy test by HCG hormone10. Estimation of Glycosylated haemoglobin
