Protocols and Applications in Enzymology

Offers thorough instruction on the experimental procedures and applications of enzymology across biochemistry, microbiology, biotechnology, and allied fields

Seema Anil Belorkar (Author), Sudisha Jogaiah (Author)

9780323912686

Paperback / softback, published 24 November 2021

266 pages, 60 illustrations (30 in full color)
23.5 x 19 x 1.8 cm, 0.54 kg

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.

1. Introduction Enzymes-Past, present and Future 2. Distribution and diversity in microbial enzymes 3. Screening of potential microbes for enzymes of industrial significance 4. Solid state fermentations and SMF for enzyme production 5. Laboratory methods for enzyme assays 6. Structure and functions of enzyme kinetics 7. Protocols of important industrial enzymes 8. Strategies to improve enzyme activity for industrial processes 9. Scope and relevance of Industrial applications 10. Agro-industrial wastes for enzyme production 11. Significance of enzymes and their agricultural applications 12. Biotechnological applications of enzymes and future prospective 13. Conclusions

Subject Areas: Molecular biology [PSD]