Proteomics in Biology, Part B

This new volume in the Methods in Enzymology series continues the legacy of this premier serial, focusing its attention on proteomics

Arun K. Shukla (Volume editor)

9780128097434

Hardback, published 1 February 2017

536 pages
22.9 x 15.1 x 3.1 cm, 1 kg

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience

Proteomics in Biology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in proteomics.

1. Protein Cysteinyl-S-Nitrosylation: Analysis and Quantification 2. Quantitative Proteomics of the E. coli Membranome 3. Comparative Analysis and Validation of Different Steps in Glycomics Studies 4. Analysis of Translocation-Competent Secretory Proteins by HDX-MS 5. Site-Specific Quantification of Lysine Acetylation Using Isotopic Labeling 6. Posttranslational Modifications and Plant–Environment Interaction 7. Identification of Posttranslational Modifications of Endogenous Chromatin Proteins From Testicular Cells by Mass Spectrometry 8. Quantitative Analysis of Yeast Checkpoint Protein Kinase Activity by Combined Mass Spectrometry Enzyme Assays 9. Retrieving Quantitative Information of Histone PTMs by Mass Spectrometry 10. Evaluating Exosome Protein Content Changes Induced by Virus Activity Using SILAC Labeling and LC-MS/MS 11. Exoproteomics of Pathogens: Analysis of Toxins and Other Virulence Factors by Proteomics 12. Integrated and Quantitative Proteomics of Human Tumors 13 .Mass Spectrometry-Based Analysis for the Discovery and Validation of Potential Colorectal Cancer Stool Biomarkers 14. Mass Spectrometry-Based Methodology for Identification of Native Histone Variant Modifications From Mammalian Tissues and Solid Tumors 15. Drug Target Identification Using an iTRAQ-Based Quantitative Chemical Proteomics Approach—Based on a Target Profiling Study of Andrographolide 16. A Super-SILAC Strategy for the Accurate and Multiplexed Profiling of Histone Posttranslational Modifications 17. A Comparison of Two-Hybrid Approaches for Detecting Protein–Protein Interactions 18. Recent Achievements in Characterizing the Histone Code and Approaches to Integrating Epigenomics and Systems Biology 19. Rapid Proteomics to Prospect and Validate Novel Bacterial Metabolism Induced by Environmental Burden 20. Quantitation of Human Metallothionein Isoforms in Cells, Tissues, and Cerebrospinal Fluid by Mass Spectrometry 21. A Cautionary Tale on the Inclusion of Variable Posttranslational Modifications in Database-Dependent Searches of Mass Spectrometry Data 22. Kinase Assay-Linked Phosphoproteomics: Discovery of Direct Kinase Substrates

Subject Areas: Molecular biology [PSD], Biochemistry [PSB], DNA & Genome [PSAK1], Biophysics [PHVN]