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Proteomic Profiling and Analytical Chemistry
The Crossroads
By providing an overview and understanding of the analytical chemistry tools applicable to proteomic profiling and validation experiments, this book bridges the gap between overly specialized courses and books in mass spectrometry, proteomics, and analytical chemistry, helping researchers with an analytical chemistry background to break into the proteomics field
Pawel Ciborowski (Edited by), Jerzy Silberring (Edited by)
9780444636881
Paperback / softback, published 23 March 2016
298 pages
22.9 x 15.1 x 2 cm, 0.34 kg
Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study. The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
Preface
1. Introduction
Jerzy Silberring and Pawel Ciborowski
2. Biomolecules
Ariel Burns, Pawel Olszowy and Pawel Ciborowski
3. General Strategies for Proteomic Sample Preparation
Piotr Suder, Peter Novak, Vladimir Havlicek and Anna Bodzon-Kulakowska
4. Protein Extraction and Precipitation
Petr Novak and Vladimir Havlicek
5. Online and Offline Sample Fractionation
Marek Smoluch, Przemyslaw Mielczarek, Anna Drabik and Jerzy Silberring
6. Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Preparation
Jayme Wiederin and Pawel Ciborowski
7. Gel Electrophoresis
Anna Drabik, Anna Bodzon-Kulakowska and Jerzy Silberring
8. Quantitative Measurements in Proteomics: Mass Spectrometry
Anna Drabik and Jerzy Silberring
9. SWATH-MS: Data Acquisition and Analysis
Kristin Frederick and Pawel Ciborowski
10. Top-Down Proteomics
Cory Boone and Jiri Adamec
11. Proteomic Database Search and Analytical Quantification for Mass Spectrometry
Melinda Wojtkiewicz, Jayme Wiederin and Pawel Ciborowski
12. Design and Statistical Analysis of Mass-Spectrometry-Based Quantitative Proteomics Data
Fang Yu, Fang Qiu and Jane Meza
13: Principles of Analytical Validation
Jo-Ellyn McMillan
14.Validation in Proteomics and Regulatory Affairs
Jerzy Silberring, Melinda Wojtkiewicz and Pawel Ciborowski
Subject Areas: Biochemistry [PSB], Analytical chemistry [PNF]
