Protein Liquid Chromatography

M. Kastner (Author)

9780444502117, Elsevier Science

Paperback, published 14 December 1999

933 pages
23.3 x 15.6 x 5.6 cm, 1.27 kg

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Part and chapter headings:Individual Chromatographic Modes. Ion exchange chromatography (P.H. Roos). Size exclusion chromatography (M. R&ouml
gner). Reversed-phase chromatography (H. Schl&uuml
ter). Hydrophobic interaction chromatography (L.R. Jacob). Hydroxyapatite chromatography (R. Luka&ccaron
in, W.R. Deppert). Immobilized metal ion affinity chromatography (M. Kastner). Chromatofocusing (R. Luka&ccaron
in, W.R. Deppert). Dye-ligand affinity chromatography (J. Kirchberger, H.-J. B&ouml
hme). Immobilized artificial membrane chromatography (K. Hauer et al.). Liquid-liquid partition chromatography (U.-B. Hansson, C. Wingren). Displacement chromatography (H. Schl&uuml
ter, J. Jankowski). Various Target Protein Classes. Integral membrane proteins (G. Welling, S. Welling-Wester). Recombinant proteins (O. Huber, M. Huber-Wunderlich). DNA-binding proteins (M. Velleman). Lectins and glycoconjugates (H.-J. Gabius). Covalent chromatography (D. Whitney). Cell affinity chromatography (D.D. Putnam et al.). Miscellaneous Chromatographic Aspects. Coupling reactions (E. M&uuml
ller). Immobilization of nucleotides (E. M&uuml
ller, M. Morr). Miscellaneous biospecific affinity gels (A.M. Williams). Scale-up of downstream processing (J. Walter). Chromatography on porous matrices (J. Kruip). Chromatography using Strep-tag affinity peptides (H. N. M&uuml
ller, T.G.M. Schmidt). Buffers and additives (W.R. Deppert, R. Luka&ccaron
in). Chromatography software (T. Steger-Hartmann). Supplement. Abbreviations used in this book. Directory of manufacturers and suppliers. Subject index.

Subject Areas: Environmental science, engineering & technology [TQ], Proteins [PSBC], Polymer chemistry [PNNP], Chromatography [PNFC], Chemistry [PN]