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Protein Aggregation in Bacteria
Functional and Structural Properties of Inclusion Bodies in Bacterial Cells
Silvia Maria Doglia (Edited by), S Doglia (Author), Marina Lotti (Edited by)
9781118448526, Wiley
Hardback, published 8 August 2014
288 pages
24.4 x 16.3 x 2 cm, 0.567 kg
Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies—and on their use in biotechnological and medical applications The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications. Protein Aggregation in Bacteria covers: Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science.
Contributors vii Preface xi Introduction to the Wiley Series in Protein and Peptide Science xiii 1 Fundamentals of Protein Folding 1 2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63 3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77 4 Inclusion Bodies in the Study of Amyloid Aggregation 93 5 Protein Aggregation in Unicellular Eukaryotes 117 6 Structural Properties of Bacterial Inclusion Bodies 151 7 Residue-Specific Structural Studies of Inclusion Bodies 181 8 Biomedical Applications of Bacterial Inclusion Bodies 203 9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221 10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247 Index 263
Vladimir N. Uversky
Rayees U.H. Mattoo and Pierre Goloubinoff
Ario de Marco
Anna Villar-Piqué and Salvador Ventura
Marina Caldara, Joris Winderickx, and Vanessa Franssens
Antonino Natalello, Diletta Ami, and Silvia Maria Doglia
Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang
Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde
Marina Lotti and Loredano Pollegioni
Bernd Nidetzky
Subject Areas: Chemistry [PN]
