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Practical Forensic Microscopy
A Laboratory Manual
Barbara P. Wheeler (Author)
9781119154495, Wiley
Hardback, published 8 April 2021
496 pages
25.6 x 18.5 x 2.8 cm, 1.157 kg
An applied approach to teaching forensic microscopy in educational settings, featuring new experiments and an up-to-date overview of the field Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a unique resource that brings the microscopic procedures used by real-world forensic investigators to the college laboratory, providing hands-on knowledge of the microscopes and microscopic techniques used in the field. Presenting a balanced, skills-based approach to the subject, this student-friendly lab manual contains dozens of experiments designed to cover the various microscopic evidence disciplines, including examinations of fingerprints, firearm, toolmark, shoeprint and tire impressions, gunshots, fibers, soil, glass breakage, drugs, semen, and human hair. The second edition includes revised and updated experiments that reflect current technologies and techniques used in forensic science, including new experiments examining plastic film, food condiments, feathers, building materials, explosive residue, cigarette butts and more. Each chapter includes a list of simple objectives for the experiment, a general overview of the topic, further readings, and selected references. The manual contains worksheets and templates for students to use when compiling analytical results. The concluding chapter features an innovative case scenario that requires students to analyze items of evidence, complete a laboratory report, reach a conclusion, and present their findings. This popular lab manual: Written by an author with years of academic and professional experience, Practical Forensic Microscopy: A Laboratory Manual, 2nd Edition, is a must-have companion for any college-level forensic science course with a laboratory component, and is a useful supplement for related courses that cover microscopy and the principles of forensic lab procedures.
Preface ix Acknowledgments xi Laboratory Safety xiii Microscope Maintenance xv The Micro Kit xvii About the Companion Website xix Experiments 1 1 Stereomicroscope 3 Experiment 1A: Familiarization with the Stereomicroscope 5 2 Compound Light Microscope 13 Experiment 2A: Familiarization with the Compound Light Microscope 15 Experiment 2B: Measurements Using the Ocular Micrometer 23 Experiment 2C: Microscopic Mounting Techniques 29 Experiment 2D: Determining Refractive Index 35 3 Polarized Light Microscope 47 Experiment 3A: Familiarization with the Polarized Light Microscope 49 Experiment 3B: Determining Refractive Index of Anisotropic Materials 57 Experiment 3C: Determining Sign of Elongation and Birefringence 63 4 Fluorescence Microscope 69 Experiment 4A: Familiarization with the Fluorescence Microscope 71 5 Phase Contrast Microscope 77 Experiment 5A: Familiarization with the Phase Contrast Microscope 79 Application Experiments 85 6 Experiment 6: Physical Match Examinations 87 7 Experiment 7: Construction Examinations of Evidence 93 8 Experiment 8: Lamp Filament Examinations 103 9 Experiment 9: Fingerprint Examination and Comparison 113 10 Experiment 10: Tool Mark Examinations 121 11 Experiment 11: Firearms Examinations 127 Experiment 11A: Gunshot Residue Examinations 135 12 Experiment 12: Shoe and Tire Impression Examinations 145 13 Experiment 13: Botanical Examinations 151 14 Experiment 14: Paint Examinations 159 15 Experiment 15: Cigarette Butt Examinations 165 16 Experiment 16: Document Examinations 173 17 Experiment 17: Hair Examinations 183 Experiment 17A: Animal Hair Examinations 195 Experiment 17B: Determination of Racial and Somatic Origin Characteristics of Human Hair 205 Experiment 17C: Human Hair Examinations and Comparisons 213 Experiment 17D: Evaluation of Human Hair for DNA 223 18 Experiment 18: Feather Examinations 231 19 Experiment 19: Glass Breakage Determinations 239 Experiment 19A: Glass Examinations 247 20 Experiment 20: Fiber Examinations 257 Experiment 20A: Natural Fiber Examinations 265 Experiment 20B: Man-made Fiber Examinations 279 Experiment 20C: Fiber Comparisons 289 21 Experiment 21: Soil Examinations 295 Experiment 21A: Identification of Minerals in Soil 303 22 Experiment 22: Microchemical Testing – Inorganic Ions 317 23 Experiment 23: Building Material Examinations 329 24 Experiment 24: Explosive Residue Examinations 337 25 Experiment 25: Food Condiment Examinations 345 26 Experiment 26: Plastic Film Examinations 355 27 Experiment 27: Microscopic Examination of Controlled Substances 363 28 Experiment 28: Semen Examinations 377 Application Experiments, Instrumental Microscopy 383 29 Experiment 29: Fourier Transform Infrared Microspectrometry Examinations 385 30 Experiment 30: UV-Visible-NIR Microspectrophotometry Examinations 395 31 Experiment 31: Thermal Microscopy Examinations 405 32 Experiment 32: Scanning Electron Microscopy Examinations 413 33 Experiment 33: Case Study Scenario 423 Appendices 429 A Optical Properties of Natural Fibers 431 B Optical Properties of Man-made Fibers 433 C Michel-Lévy Chart 437 D Dispersion Staining Graph 439 E Circle Template 441 Glossary of Microscopy Terms 443 Index 453
Subject Areas: Chemistry [PN]
