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Platelet Proteomics
Principles, Analysis, and Applications
Ángel García-Alonso (Edited by), García (Author), Yotis Senis (Edited by)
9780470463376, Wiley
Hardback, published 10 May 2011
432 pages
24.1 x 16.3 x 2.9 cm, 0.78 kg
"Experienced researchers looking for an up-to-date and comprehensive review of this rapidly developing field will find this book a valuable resource.” (American Society for Mass Spectrometry, 13 December 2011)
The purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets. The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, systems biology, and their applications to platelet-related diseases.
Foreword xiii Preface xv Acronyms xvii Part I General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics 1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis 3 2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research 27 3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research 67 Part II Analysis of The Platelet Proteome: Global Approaches and Subproteomes 4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies 89 5 The Platelet Membrane Proteome 111 6 Proteomics of Platelet Granules, Organelles, and Releasate 139 7 The Platelet Microparticle Proteome 159 8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome 185 Part III Integrated “Omics” and Application to Disease 9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome 209 10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders 231 11 Platelet Functional Genomics 253 12 Systems Biology to Study Platelet-Related Bleeding Disorders 285 13 Platelet Proteomics in Transfusion Medicine 321 14 Cardiovascular Proteomics 341 Index 381
Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins
Julia Maria Burkhart and Albert Sickmann
Maria Zellner and Rudolf Oehler
Ángel García
Yotis A. Senis
James P. McRedmond
David M. Smalley
Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joël Vandekerckhove, and Kris Gevaert
Michael G. Tomlinson
Dmitri V. Gnatenko and Wadie F. Bahou
Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn
Jan-Willem N. Akkerman and Bernard De Bono
Thomas Thiele, Leif Steil, Uwe Völker, and Andreas Greinacher
Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria Álvarez-Llamas
Subject Areas: Chemistry [PN]
