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    Plant Biochemistry
    Techniques and Procedures

    G. Nagaraj (Author)

    9789383305940

    Hardback, published 15 June 2015

    512 pages
    25 x 15 x 1.5 cm, 0.99 kg

    This book is designed to aid analysts in the analysis of plant samples. It is divided into two parts. The first part covers the various biochemical techniques used in the analysis, including the theoretical foundations and the mechanisms of the required equipment. The necessity and scope of the instruments, as well as their usefulness, are also discussed. The second part focuses on specific analytical methods, including those for sugars, amino acids, proteins, fats, enzymes, and their components, as well as nucleic acids, vitamins, antinutrients (toxicants), and minerals. Additionally, information on alkaloid and pesticide residue analysis is provided, with multiple methods given for each category. These methods include reagents, apparatus, and step-by-step procedures, with final calculations for determining the concentration of constituents included.

    • Part I. Analytical Techniques
    • 1. Introduction
    • 2. Chromatography
    • 3. Paper Chromatography
    • 4. Column Chromatography
    • 5. Thin Layer Chromatography
    • 6. Electrophoresis
    • 7. Ion Exchange Chromatography
    • 8. Gel-filtration Chromatography
    • 9. Solvent Extraction
    • 10. Dialysis, Ultra Filtration and Lyophilisation
    • 11. Centrifugation
    • 12. Gas Liquid Chromatography
    • 13. High Pressure Liquid Chromatography
    • 14. GC/MS and LC/MS
    • 15. Spectrophotometry
    • 16. Infra Red Spectroscopy
    • 17. Flame Photometry
    • 18. Atomic-absorption Spectroscopy
    • 19. Inductively Coupled Plasma Atomic Emission Spectroscopy
    • 20. NMR Spectroscopy
    • 21. Radiochemical Methods
    • 22. Mass Spectrometry
    • 23. Polarimetry
    • 24. Conductimetry
    • 25. Potentiometry
    • 26. ELISA and Radioimmunoassay (RIA)
    • 27. Gravimetric Analysis
    • 28. Volumetric Analysis
    • 29. Amplification and Sequencing of Nucleic Acids.
    • Part II. Analytical Methods and Procedures
    • 1. Methods of Analysis
    • 2. Carbohydrates and their Methods of Analysis
    • 3. Fats/Oils/Lipophilic Constituents
    • 4. Amino Acids and Proteins
    • 5. Enzymes
    • 6. Nucleic Acids
    • 7. Vitamines
    • 8. Phenolics and Antinutrients
    • 9. Toxins
    • 10. Alkaloids
    • 11. Plant Nutrients
    • 12. Plant Pigments
    • 13. Pesticide Residue Analysis
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