PCR Guru
An Ultimate Benchtop Reference for Molecular Biologists

Provides researchers in molecular biology with a handy reference to help solve challenging problems in PCR setup and optimization

Ayaz Najafov (Author), Gerta Hoxhaj (Author)

9780128042311

Paperback / softback, published 9 December 2016

104 pages
22.9 x 15.1 x 0.8 cm, 0.2 kg

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR.

1. Introduction2. Procedure3. Good PCR Practices4. Troubleshooting5. Tips6. Special CasesAppendix A. Agarose Gel Electrophoresis ProtocolAppendix B. Silver Staining of DNA GelsAppendix C. Plasmid Miniprep ProtocolAppendix D. Restriction Enzyme Digestion ProtocolAppendix E. Protocol for DNA Concentration Estimation by using Hoechst 33258Appendix F. FormulasAppendix G. Primers for Common Promoters, Housekeeping Genes, Reporters, etc.Appendix H. TagsAppendix I. Thermostable DNA polymerasesAppendix J. Example of a Daily PCR LogAppendix K. Common Buffers and SolutionsAppendix L. Useful numbersAppendix M. Yeast colony PCR

Subject Areas: Genetics [non-medical PSAK]