Optogenetics
From Neuronal Function to Mapping and Disease Biology

This book provides applications for the revolutionary technique of optogenetics, which combines genetic and optical methods to observe the function of neurons.

Krishnarao Appasani (Edited by)

9781107053014, Cambridge University Press

Hardback, published 27 April 2017

502 pages, 65 b/w illus. 37 colour illus. 6 tables
25.3 x 17.9 x 2.9 cm, 1.2 kg

'Both the expert and novice, alike, will find this to be a valuable resource that covers multiple aspects of the Optogenetics field, from its history through to current scientific and potential clinical applications.' Michelle M. Sidor, Novartis Institutes for Biomedical Research, Massachusetts

Discovered little more than a decade ago, optogenetics - a revolutionary technique combining genetic and optical methods to observe and control the function of neurons - is now a widely used research tool. Optogenetics-driven research has led to insights into Parkinson's disease and other neurological and psychiatric disorders. With contributions from leaders and innovators from both academia and industry, this volume explores the discovery and application of optogenetics, from the basic science to its potential clinical use. Chapters cover a range of optogenetics applications, including for brain circuits, plasticity, memory, learning, sleep, vision and neurodegenerative and neuropsychiatric diseases. Providing authoritative coverage of the huge potential that optogenetics research carries, this is an ideal resource for researchers and graduate students, as well as for those working in the biotechnology and pharmaceutical industries and in a clinical setting.

List of contributors
Foreword
Preface
List of abbreviations
Part I. Optogenetics in Model Organisms: 1. Introduction to optogenetics: from neuronal function to mapping and disease biology
2. Uncovering key neurons for manipulation in mammals
3. From connectome to function: using optogenetics to shed light on the Caenorhabditis elegans nervous system
4. From synapse to behaviour: optogenetic tools for investigation of the Caenorhabditis elegans nervous system
5. Using optogenetics in vivo to stimulate regeneration in Xenopus laevis
Part II. Opsin Biology, Tools and Technology Platform: 6. Sodium and engineered potassium light-driven pumps
7. Simultaneous electrophysiology and optogenetic stimulation methods
8. Role of electrical activity in horizontal axon growth in the developing cortex: a time-lapse study using optogenetic stimulation
9. Development of an optogenetic tool to regulate protein stability in vivo
10. Photo-activatable nucleotide cyclases for synthetic photobiology applications
11. Bioluminescence activation of light-sensing molecules
Part III. Optogenetics in Neurobiology, Brain Circuits and Plasticity: 12. Optogenetics for neurological disorders: what is a path to the clinic?
13. Optogenetic control of astroglia
14. Optogenetics for neurohormones and neuropeptides: focus on oxytocin
15. Optogenetic approaches to investigate brain circuits
16. Optogenetic mapping of neuronal connections and their plasticity
Part IV. Optogenetics in Learning, Neuro-psychiatric Diseases and Behavior: 17. Optogenetics to study reward learning and addiction
18. Optogenetics and the dissection of neural circuits underlying depression and substance-use disorders
19. Optogenetics research in behavioral neuroscience: insights into the brain basis of reward learning and goal-directed behavior
20. An optogenetic approach to treat epilepsy
21. Using optogenetics and stem cells-derived neural engraftment techniques to restore lost motor function
Part V. Optogenetics in Vision Restoration and Memory: 22. Optogenetics in treating retinal disease
23. Optogenetics for vision recovery: from traditional to designer optogenetic tools
24. A promise of vision restoration
25. Holographic Optical Neural Interfacing (HONI) with retinal neurons
26. Strategies for restoring vision by transducing a channelrhodopsin gene into retinal ganglion cells
27. Optogenetic dissection of a top-down prefrontal-to-hippocampus memory circuit
Part VI. Optogenetics in Sleep, Prosthetics and Epigenetics of Neurodegenerative Diseases: 28. Optogenetic dissection of sleep-wake control: evidence for a thalamic control of sleep architecture
29. Optogenetics and auditory implants
30. Optogenetic stimulation for cochlear prosthetics
31. The role of amino acids in neurodegenerative and addictive diseases
32. Applications of combinations of deep brain stimulation and optogenetics: ethical considerations: an epilogue.

Subject Areas: Applied optics [TTB], Genetic engineering [TCBG], Biotechnology [TCB], Neurosciences [PSAN], Genetics [non-medical PSAK], Biophysics [PHVN], Neurology & clinical neurophysiology [MJN], Medical genetics [MFN], Experimental psychology [JML]