Molecular Imaging
FRET Microscopy and Spectroscopy

Ammasi Periasamy (Edited by), Richard Day (Edited by)

9780195177206, Elsevier Science

Hardback, published 10 March 2008

312 pages
26 x 18.4 x 2.3 cm, 1.18 kg

"This is a gold mine of information for those interested in using FRET microscopy to study protein-protein interactions. A major strength of this book is that it is focused and yet comprehensive...The book is clearly written, carefully edited, and engaging...It will be a valued resource and practical guide for years to come...5 Stars! (Highest Rating)" --Doody's

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided.

The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Proteins and the Flow of Information in Cellular Function
Basics of Fluorescence and FRET
An Introducrion to Filters and Mirrors for FRET
FRET Imaging in the Wide-field Microscope
Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes
Multiphoton FRET Microscopy for Protein Localization in Tissue
Single-Molecule FRET
FRET Measurements using Multispectral Imaging
Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers
Streak FLIM: A Novel Technology for Quantitative FRET Imaging
Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization
Bioluminescence RET (BRET): Techniques and Potential
Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy
Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy
Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization

Subject Areas: Molecular biology [PSD]