Modifications and Targeting of Protein Termini Part A

Edited by some of the best scientists in the field, this series presents the latest updates in the field of enzymology

Thomas Arnesen (Volume editor)

9780443157721, Elsevier Science

Hardback, published 24 May 2023

338 pages
22.9 x 15.2 x 2.4 cm, 0.45 kg

Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.

1. Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells Bernd Bukau, Jiri Koubek, Manuel Günnigmann and Günter Kramer 2. Deformylation of nascent chains on the ribosome Marina V. Rodnina, Lena Bögeholz, Evan Mercier and Wolfgang Wintermeyer 3. Optimizing purification and activity assays of N-terminal methyltransferase complexes Christine Schaner Tooley, Haley Parker and John Tooley 4. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods Rong Huang and Ying Meng 5. Biochemical and structural analysis of N-myristoyltransferase-mediated protein tagging Thierry Meinnel, Carmela Giglione, Paul Monassa, Frédéric Rivière, Cyril Dian and Frédéric Frottin 6. Kinetic and catalytic features of N-myristoyltransferases Thierry Meinnel and Carmela Giglione 7. Detecting N-terminal myristoylation using metabolic Alk12 labeling Hening Lin and Yilai Xu 8. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities Iris Finkemeier, Jürgen Eirich, Julia Sindlinger, Stefan Schön and Dirk Schwarzer 9. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms Kris Gevaert, Annelies Bogaert, Tessa Van de Steene, Marnik Vuylsteke and Sven Eyckerman 10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification, interaction studies and enzymatic assays Ruth Geiss-Friedlander, Laura Donzelli and Oguz Bolgi

Subject Areas: Molecular biology [PSD], Biochemistry [PSB]