Methods of Adipose Tissue Biology Part B
Methods of Adipose Tissue Biology

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field.

Ormond A Macdougald (Volume editor)

9780128002803

Hardback, published 10 April 2014

344 pages
22.9 x 15.1 x 2.4 cm, 0.57 kg

Approx.328 pages

1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover
3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation
6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry
7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity
9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes
10. Measurement of Lipolysis
11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics
12. Methods for Performing Lipidomics in White Adipose Tissue
13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time
14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes
15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis
16. Evaluation of Protein Phosphorylation during Adipogenesis

Subject Areas: Cellular biology [cytology PSF], Biochemistry [PSB], Cellular physiology [MFGC]