Methods of Adipose Tissue Biology Part A

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field.

Ormond A Macdougald (Volume editor)

9780124116191

Hardback, published 17 March 2014

360 pages
22.9 x 15.1 x 2.5 cm, 0.61 kg

Approx.342 pages

1. Adipocyte-Specific Transgenic and Knockout Models
2. Imaging White Adipose Tissue With Confocal Microscopy
3. Isolation and Study of Adipocyte Precursors
4. Imaging of Adipose Tissue
5. Adipose Tissue Angiogenesis Assay
6. Quantifying Size and Number of Adipocytes in Adipose Tissue
7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo
8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)
9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin
10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes
11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue
12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion
13. Isolation and Quantitation of Adiponectin Higher Order Complexes
14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq
15. Analysis and Isolation of Adipocytes by Flow Cytometry
16. Flow Cytometry Analyses of Adipose Tissue Macrophages

Subject Areas: Cellular biology [cytology PSF], Histology [MFCH]