Methods in Stem Cell Biology - Part B

Edited by one of the best scientists in the field, this series presents the latest updates in the field of cell biology

Ilio Vitale (Volume editor), Gwenola Manic (Volume editor), Lorenzo Galluzzi (Volume editor)

9780323900188, Elsevier Science

Hardback, published 9 August 2022

260 pages
22.9 x 15.2 x 2.2 cm, 0.7 kg

Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New Therapies Xiaoyan Jiang 2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR Guillermo Mazzolini 3. Reverse Phase Protein Array in cancer stem cells Michele Signore 4. Isolation of cancer stem cells from squamous cell carcinoma Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC), 5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells Niccolò Bartalucci 6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity Paola Marcato 7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs) Angela Gritti 8. Immunohistochemistry-based analysis of EMT markers in mammary tumors Diwakar Pattabiraman 9. Immunofluorescence staining of colorectal cancer patient-derived organoids Joseph L. Regan 10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry Michael A. Rieger 11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells Bin Wang Sr., Yingying Lai and Xi Zheng 12. Methods to isolate adipose tissue-derived stem cells Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco 13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells Antonella Sistigu

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC]