Methods in iPSC Technology

A timely and important collection of different methods used for induced pluripotent stem cells formation, maintenance, expansion and differentiation

Alexander Birbrair (Edited by)

9780323857666, Elsevier Science

Paperback / softback, published 9 June 2021

370 pages, 60 illustrations (40 in full color)
23.5 x 19 x 2.4 cm, 0.72 kg

The series Advances in Stem Cell Biology is a timely and expansive collection of comprehensive information and new discoveries in the field of stem cell biology.

Methods in iPSC Technology, Volume 9 addresses the different methods used for induced pluripotent stem cell (iPSC) formation, maintenance, expansion, and differentiation.

The ability to reprogram different cell types to iPSCs offers an opportunity to generate pluripotent patient-specific cell lines that can help in the understanding of multiple human disorders. This volume addresses a variety of current methods used in the generation and manipulation of iPSCs, such as magnetic nanoparticles, piggyBac vectors, lentiviral vectors, bioscaffolds, somatic cell nuclear transfer, CRISPR/Cas9, bacteria, and much more.

This volume is written for researchers and scientists interested in stem cell therapy, cell biology, regenerative medicine, and organ transplantation and is contributed by world-renowned authors in the field.

1. Current Reprogramming Methods to Generate High Quality iPSCs
2. Using Magnetic Nanoparticles in iPSCs
3. PiggyBac Vectors in Pluripotent Stem Cell Research and Applications
4. Lentiviral Vectors as The Delivery Vehicles for Transduction into iPSCs: Shortcomings and Benefits
5. Decellularized Liver Extracellular Matrix for iPSC-based Liver Engineering
6. Combining Bioscaffolds and iPCSs in the Treatment of Neural Trauma and Alzheimer’s disease
7. Emerging Strategies for Scalable Human Induced Pluripotent Stem Cell and Expansion and Differentiation
8. One Plus One Could be Greater than Two: Combining the Powers of Somatic Cell Nuclear Transfer with Yamanaka’s Factors in Generating Clinical Grade Human Pluripotent Stem Cells
9. Bacteria to Form Induced Pluripotent Stem Cells
10. CRISPR/Cas9 Technologies to Manipulate Human Induced Pluripotent Stem Cells
11. Scalable Expansion of Human Pluripotent Stem Cells for Biomanufacturing Cellular Therapeutics
12. Xeno-Free Cultivation of Human Induced Pluripotent Stem Cells for Clinical Applications

Subject Areas: Life sciences: general issues [PSA]