Metabolic Analysis Using Stable Isotopes

Continues the legacy of this premier serial with chapters authored by leaders in the field

Christian Metallo (Volume editor)

9780128022931

Hardback, published 8 September 2015

402 pages
22.9 x 15.1 x 2.7 cm, 0.79 kg

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience

Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes.

1. Studies of Metabolism Using 13C MRS of Hyperpolarized Probes
Myriam M. Chaumeil,Chloé Najac and Sabrina M. Ronen
2. Hyperpolarized 13C Magnetic Resonance and Its Use in Metabolic Assessment of Cultured Cells and Perfused Organs
Lloyd Lumata, Chendong Yang, Mukundan Ragavan, Nicholas Carpenter, Ralph J. DeBerardinis and Matthew E. Merritt
3. Metabolic Tracing using Stable Isotope-Labelled Substrates and Mass  Spectrometry in the Perfused Mouse Heart
Matthieu Ruiz, Roselle Gélinas, Fanny Vaillant, Benjamin Lauzier and Christine Des        Rosiers
4. Probing Metabolism in the Intact Retina Using Stable Isotope Tracers
Jianhai Du, Jonathan D. Linton and James B. Hurley
5. Analysis of Cell Metabolism Using LC-MS and Isotope Tracers
Gillian M Mackay, Liang Zheng, Niels JF van den Broek and Eyal Gottlieb
6. Analysis of Fatty Acid Metabolism Using Stable Isotope Tracers and Mass Spectrometry
Sergey Tumanov, Vinay Bulusu, and Jurre J. Kamphorst
7. Dynamic Proteomics: In Vivo Proteome-Wide Measurement of Protein Kinetics Using Metabolic Labeling
W.E. Holmes, T.E. Angel, K.W. Li and M.K. Hellerstein
8. Non-targeted Tracer Fate Detection
Daniel Weindl, Andre Wegner and Karsten Hiller
9. Isotopomer Spectral Analysis: Utilizing Nonlinear Models in Isotopic Flux        Studies
Joanne K Kelleher and Gary B. Nickol
10. Effect of Error Propagation in Stable Isotope Tracer Studies: An Approach for Estimating Impact on Apparent Biochemical Flux.
Stephen F. Previs, Kithsiri Herath, Jose Castro-Perez, Ablatt Mahsut, Haihong Zhou, David G. McLaren, Vinit Shah, Rory J. Rohm, Steven J. Stout, Wendy Zhong, Sheng-    Ping Wang, Douglas G. Johns, Brian K. Hubbard, Michele A. Cleary and Thomas P.     Roddy

Subject Areas: Biochemistry [PSB], Analytical chemistry [PNF], Biophysics [PHVN], Science: general issues [PD]