Membrane Protein Purification and Crystallization
A Practical Guide

Carola Hunte (Edited by), Gebhard von Jagow (Edited by), Hermann Schagger (Edited by)

9780123617767

Paperback / softback, published 5 January 2003

316 pages
23.4 x 19 x 2.4 cm, 0.59 kg

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

I. Strategies and Techniques Purification Strategies for Membrane Proteins Chromatographic Techniques and Basic Operations in Membrane Protein Purification Production and purification of recombinant membrane proteins SDS Electrophoresis Techniques Blue-Native Electrophoresis Preparative Isoelectric Focussing Membrane protein crystallization

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Proteins [PSBC], Biochemistry [PSB], Biophysics [PHVN]