Measuring in vivo Oxidative Damage
A Practical Approach

J. Lunec (Edited by), J Lunec (Author), H. R. Griffiths (Edited by)

9780471818489, Wiley

Hardback, published 27 March 2000

194 pages
23.8 x 16 x 2 cm, 0.397 kg

"This book is an ideal practical reference work for the clinical scientist and is recommended for all laboratories in hospitals and research institutions which are actively involved in analytical work." (Clinical Laboratory International, June 2000)

Seit ungefähr 15 Jahren weiß man, daß freie Sauerstoffradikale eine Schlüsselrolle bei der Entstehung bestimmter Krankheiten (Arteriosklerose, chronische Entzündungen) spielen. Dieses Handbuch der Methodologie der biologischen Analytik auf dem Gebiet der oxidativen Schädigungen richtet sich an klinische Wissenschaftler, die eine geeignete in-vivo-Methode suchen. Übersichtlich in fünf Teile unterteilt, ist das Buch ein praktischer Begleiter im Laboralltag. (03/00)

CHROMATOGRAPHIC PROCEDURES.

Lipid Peroxidation Determination by HPLC (R. Bevan).

Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.).

The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.).

MEASUREMENT OF 8-OXO DEOXYGUANOSINE.

Measurement of 8-Oxo-2' -deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography-Electrochemical Detection (M. Evans).

Immunochemical Detection of 8-Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert).

Urinary Measurement of 8-OxodG (8-Oxo-2' -deoxyguanosine) (H. Poulsen, et al.).

CELLULAR-BASED METHODS.

Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins).

Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe).

MOLECULAR-BASED ASSAYS.

A ¯32P-Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld).

Mapping Reactive Oxygen-Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman).

ANTIOXIDANT ACTIVITY.

Measurements of Plasma Antioxidant Activity (S. Maxwell).

Index.

Subject Areas: Chemistry [PN]