Mass Spectrometry
Techniques for Structural Characterization of Glycans

This short-format reference presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrate molecules, thus providing the relevant research necessary for advances in this area of study

Michael A. Madson (Author)

9780128041291

Hardback, published 23 May 2016

86 pages
22.9 x 15.1 x 1.6 cm, 0.32 kg

Approx.78 pages

• Introduction• Monosaccharide Composition Analysis• Elemental Analysis of MS Ions• Derivatization Techniques• Hydride Addition to Sulfate and phosphate- Chemistry• InstrumentationMALDI-TOF MSAPI 2000 MSICP ESI TOF MS• Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters• Ester Substitution MS MethodThe Protocol for the Hydride Addition to the ethanol extract of banana fruitExample of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan• Glycan Isolation Methods• Other Glycan isolation Methods• Monosaccharide AnalysisAcid Hydrolysis• Mass Spectral Carbohydrate Structure Determination MethodMSMS/MS (MS2)MSnProposing Initial Structure Refining Methods for Determining Exact Mass StructureMS Instrumentation Requirements for Discovering Whole Molecule IonsDerivatization Method and Sulfate/Phosphate Discernment Method• References

Subject Areas: Organic chemistry [PNN], Analytical chemistry [PNF], Scientific equipment, experiments & techniques [PDN]