Marrow Stromal Cell Culture

Concise handbook on crucial technique for clinicians and scientists working on bone and blood.

Jon N. Beresford (Edited by), Maureen E. Owen (Edited by)

9780521589789, Cambridge University Press

Paperback, published 16 April 1998

168 pages, 26 b/w illus.
22.9 x 15.3 x 1.1 cm, 0.28 kg

' … the book is extremely helpful for those starting to work in this area, as well as for the advanced hematologist who is interested in stroma-stem cell type investigations.' R. E. Ploemacher

The first book of its kind on marrow stromal cells, and written by leading experts in the field, this concise handbook aims to provide all that is needed to those new to this relatively young area of research. It provides a historical perspective and a summary of present knowledge of the marrow stromal lineages which are currently best understood. Detailed protocols for the isolation, culture and characterization of marrow stromal cell types from human and other animal species are given. The breadth and depth of the subject matter included makes the book equally suitable for new researchers and the more experienced investigator in this rapidly expanding field, including clinicians and bioscientists.

Preface to the series
1. The marrow stromal cell system Maureen E. Owen
2. The bone marrow stroma in vivo: ontogeny, structure, cellular composition and changes in disease Paolo Bianco and Mara Riminucci
3. Isolation, purification and in vitro manipulation of human bone marrow stromal precursor cells Stan Gronthos, Stephen E. Graves and Paul J. Simmons
4. Isolation and culture of human bone-derived cells Roger Gundle, Karina Stewart, Joanne Screen and Jon N. Beresford
5. Marrow stromal adipocytes Jeffrey M. Gimble
6. Osteoblast lineage in experimental animals Jane E. Aubin and Alexa Herbertson
7. Chondrocyte culture Ranieri Cancedda, Fiorella Descalzi Cancedda and Beatrice Dozin
8. Osteogenic potential of vascular pericytes Ana M. Schor and Ann E. Canfield
Index.

Subject Areas: Cellular biology [cytology PSF], Scientific equipment, experiments & techniques [PDN]