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Manual of Techniques in Invertebrate Pathology
Now including pathogens of additional invertebrates, the 2nd edition provides essential coverage of step-by-step protocols for the isolation, identification, propagation, bioassay and preservation of entomopathogens.
Lawrence A. Lacey (Edited by)
9780123868992, Elsevier Science
Paperback / softback, published 25 April 2012
504 pages
27.6 x 21.5 x 3.1 cm, 1.28 kg
Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.
Manual of Techniques in Invertebrate Pathology, 2nd edition Lawrence A. Lacey, editor 1. Initial Handling and Diagnosis of Diseased Invertebrates Lawrence A. Lacey and Leellen Solter 2. Basic Techniques in Insect Virology Karolin E. Eberle, Jörg T. Wennmann, Regina G. Kleespiesand Johannes A. Jehle 3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli. Tanja W. Fisher and Steven F. Garczynski 4. Bioassay of Bacterial Entomopathogens Against Insect Larvae Maureen O’Callaghan, Travis R. Glare and Lawrence A. Lacey 5. Bacteria for use Against Soil-Inhabitating Insects Albrecht M. Koppenhöfer, Trevor A. Jackson, and Michael G. Klein 6. Identification of Entomopathogenic Fungi Richard A. Humber 7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel 8. Mass Production of Entomopathogenic Hypocreales Stefan T. Jaronski and Mark A. Jackson 9. Methods for the Study of Entomophthorales Ann E. Hajek, Bernard Papierok, and Jørgen Eilenberg 10. Preservation of Entomopathogenic Fungal Cultures Richard A. Humber 11. Research Methods for Entomopathogenic Microsporidia and Other Protists Leellen Solter, James Becnel, and Jiri Vávra 12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance S. Patricia Stock and Heidi Goodrich-Blair 13. Pathogens and Parasites of Terrestrial Molluscs Michael J. Wilson 14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals Joel P. Siegel 15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy James J. Becnel
Subject Areas: Insects [entomology PSVT7], Zoology: Invertebrates [PSVT], Animal pathology & diseases [PSVL], Microbiology [non-medical PSG]
