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Manual of Molecular and Clinical Laboratory Immunology
2 Volume Set
John L. Schmitz (Edited by), JL Schmitz (Author), Barbara Detrick (Edited by), Maurice R. G. O'Gorman (Edited by)
9781683673996, Wiley
Hardback, published 26 December 2024
1440 pages
28.2 x 22.4 x 6.6 cm, 3.47 kg
THE authoritative guide for clinical laboratory immunology For nearly 50 years, the Manual of Molecular and Clinical Laboratory Immunology has been the premier resource for laboratories, students, and professionals involved in the clinical and technical details of diagnostic immunology testing. The 9th Edition continues its tradition of providing comprehensive clinical and technical information on the latest technologies used in medical and diagnostic immunology. Led by a world-renowned group of authors and editors, this new edition reflects substantial changes aimed at improving and updating the Manual’s utility while reflecting the significant transformations that have occurred since the last edition, including the revolution of gene editing and the widespread adoption of molecularly engineered cellular therapies. Topical highlights include: The 9th Edition of the Manual of Molecular and Clinical Laboratory Immunology serves as an invaluable resource for laboratory directors, clinicians, laboratory managers, technologists, and students. It provides critical insights into the selection, application, and interpretation of immunologic tests, offering practical guidance on troubleshooting, clinical application, and an understanding of test limitations. This comprehensive and up-to-date manual remains an essential tool for anyone involved in the diagnosis, evaluation, and management of immune-mediated and immune system-related disorders.
Volume 1 Editorial Board xi Contributors xiii Preface xxiii Author and Editor Conflicts of Interest xxv Section A Molecular Methods 1 Volume Editor: John L. Schmitz Section Editor: Eric T. Weimer 1 Introduction 3 2 Molecular Methods for Genetic Diagnosis of Inborn Errors of Immunity 5 3 Immunoglobulin and T- Cell Receptor Gene Assessment 23 Section B Immunoglobulin Methods 39 Volume Editor: Barbara Detrick Section Editor: David F. Keren 4 Introduction 41 5 Clinical Indications and Applications of Serum and Urine Protein Electrophoresis 43 6 Immunochemical Characterization of Immunoglobulins in Serum, Urine, and Cerebrospinal Fluid 62 7 Cryoglobulins and Cryofibrinogenemia 79 8 Strategy for Detecting and Following Monoclonal Gammopathies 90 Section C Complement 107 Volume Editor: Barbara Detrick Section Editor: Ashley Frazer-Abel 9 Introduction 109 10 Methods of Complement Analysis 111 11 Assessing Complement Dysregulation in the Thrombotic Microangiopathies and Ultrarare Complement- Mediated Kidney Disease 119 12 Current and Future of Complement Therapeutics and their Impact on the Clinical Immunology Laboratory 129 Section D Flow Cytometry 139 Volume Editor: Maurice R. G. O’gorman Section Editor: Paul K. Wallace 13 Introduction: Clinical Flow Cytometry Applications and Quality Control 141 14 Polychromatic Flow Cytometry 144 15 High- Sensitivity Detection of Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry 166 16 Standardized Flow Cytometry Assays for Enumerating CD34 + Hematopoietic Stem Cells 189 17 The Detection of Fetal Red Cells in Fetomaternal Hemorrhage by Flow Cytometry 213 18 Functional Flow Cytometry- Based Assays of Myeloid and Lymphoid Functions for the Diagnostic Screening of Inborn Errors of Immunity 221 19 Quality Assurance and Validation in Clinical Flow Cytometry 231 20 Future Cytometric Technologies and Applications 249 Section E Functional Cellular Assays 253 Volume Editor: Maurice R. G. O’gorman Section Editors: Miranda B. Carper And Thomas N. Denny 21 Introduction 255 22 Cryopreservation of Blood Mononuclear Cells 257 23 Functional Assays for B Cells and Antibodies 266 24 Methods for Detection of Antigen- Specific T Cells by Enzyme- Linked Immunospot Assay 277 25 Regulatory T Cell (Treg) Characterization: Phenotype, Function, and Clinical Importance of Human Treg 284 26 Measurement of Natural Killer Cell Phenotype and Activity in Humans 292 27 Functional Assays for the Diagnosis of Chronic Granulomatous Disease 304 28 Interferon Gamma Release Assays for Tuberculosis Diagnosis 318 29 Methods for Evaluating Antigen- Specific Cytotoxic T Lymphocyte Frequency and Activity 324 Section F Cytokines 333 Volume Editor: Barbara Detrick Section Editors: John J. Hooks And Timothy B. Niewold 30 Introduction 335 31 Overview of the Cytokine Assay Multiverse: Systems and Applications 337 32 Monitoring Interferons in Autoimmune Diseases and Interferonopathies 344 33 Detection of Anti-Cytokine Autoantibodies and Clinical Applications 352 Section G Laboratory Management 363 Volume Editor: John L. Schmitz Section Editor: Thomas S. Alexander 34 Licensure and Accreditation 365 35 Validation and Quality Control: General Principles and Application to the Clinical Immunology Laboratory 369 36 Quality Assurance and Quality Management of the Medical Laboratory 384 Section H Infectious Diseases Caused By Bacteria, Mycoplasmas, Chlamydiae, and Rickettsiae 393 Volume Editor: John L. Schmitz Section Editor: Christine M. Litwin 37 Introduction 395 38 Diagnostic Methods for Group A Streptococcal Infections 396 39 Laboratory Diagnosis of Syphilis 404 40 Lyme Disease, Relapsing Fever, and Leptospirosis 412 41 Immunological Tests in Tuberculosis 424 42 Mycoplasma: Serological Methods 433 43 Chlamydia Infections 438 44 The Rickettsiaceae, Anaplasmataceae, and Coxiellaceae 447 45 The Bartonellaceae, Brucellaceae, and Francisellaceae 458 Section I Mycotic and Parasitic Diseases 467 Volume Editor: John L. Schmitz Section Editor: Elitza S. Theel 46 Introduction 469 47 Immunological and Molecular Approaches for the Diagnosis of Parasitic Infections 470 48 Serological Diagnosis of Fungal Infections 489 Section J Viral Diseases 521 Volume Editor: John L. Schmitz Section Editor: Jennifer Dien Bard 49 Introduction 523 50 Herpes Simplex Virus and Varicella- Zoster Virus 524 51 Epstein- Barr Virus and Cytomegalovirus 535 52 Human Herpesviruses 6A, 6B, 7, and 8 550 53 Parvovirus B19 563 54 Severe Acute Respiratory Syndrome Coronavirus- 2 572 55 Enteroviruses 583 56 Measles, Mumps, and Rubella Viruses 592 57 Viral Hepatitis 600 58 Arboviruses 616 59 Hantavirus Infections 634 60 Rabies Virus 640 61 Human T- Cell Lymphotropic Virus Types 1 and 2 649 62 Human Immunodeficiency Virus 658 63 Bandavirus 668 Volume 2 Section K Errors Of Immunity 679 Volume Editor: Maurice R. G. O’gorman Section Editor: Roshini S. Abraham 64 Introduction to Inborn Errors of Immunity 681 65 Newborn Screening for Severe Combined Immunodeficiency 683 66 Combined Immunodeficiencies 692 67 Predominantly Antibody Deficiencies 717 68 Primary Immune Regulatory Disorders 732 69 Primary Innate Immune Defects (Including Phagocytic Disorders, TLR Defects, and NK Cell Deficiencies) 752 70 Systemic Autoinflammatory Diseases 768 71 Complement Defects 796 72 Inherited Bone Marrow Failure Syndromes: Focus on Immunodeficiency and Diagnostic Evaluation 813 73 Phenocopies of Inborn Errors of Immunity 825 Section L llergic Diseases 845 Volume Editor: Barbara Detrick Section Editor: Robert G. Hamilton 74 Introduction to Allergic Diseases 847 75 Quantitation and Standardization of Allergens 849 76 Immunological Methods in the Diagnostic Allergy Laboratory 860 77 In Vitro Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation 866 Section M Systemic Autoimmune Diseases 877 Volume Editor: Barbara Detrick Section Editor: Westley H. Reeves 78 Introduction 879 79 Standardization of Fluorescent Antinuclear Antibody Testing 881 80 Immunodiagnosis and Laboratory Assessment of Systemic Lupus Erythematosus 891 81 Immunodiagnosis of Autoimmune Myopathies 900 82 Immunodiagnosis of Scleroderma 913 83 Immunodiagnosis of Rheumatoid Arthritis 925 84 Antiphospholipid Antibody Syndrome: Clinical Manifestations and Laboratory Diagnosis 935 85 Immunodiagnosis of Systemic Vasculitis 945 86 Immunodiagnosis of Sjögren’s Disease 952 87 Diagnostic Testing for Deficiency of Adenosine Deaminase 2 962 88 Future Direction of Laboratory Testing for Autoimmune Diseases 968 Section N Organ-Localized Autoimmune Diseases 983 Volume Editor: Barbara Detrick Section Editor: Stanley J. Naides 89 Introduction 985 90 Endocrinopathies: Hashimoto Thyroiditis, Graves Disease, Hypophysitis, Addison Disease, Premature Ovarian Failure, Male Infertility, and Diabetes 987 91 Myasthenia Gravis 1014 92 Autoantibodies to Brain and Spinal Cord 1024 93 Autoantibodies to Glycolipids in Peripheral Neuropathy 1037 94 Detection of Antimitochondrial Autoantibodies in Primary Biliary Cholangitis and Liver Kidney Microsomal Antibodies in Autoimmune Hepatitis 1044 95 Cardiovascular Diseases 1054 96 Use of Laboratory Tests for Patients with Inflammatory Bowel Diseases 1071 97 Celiac Disease 1085 98 Primary Membranous Nephropathy 1090 99 Autoantibodies Directed against Erythrocytes in Autoimmune Hemolytic Anemia and Other Anemias 1098 100 Immune Thrombocytopenia 1105 101 Monitoring Autoimmune Reactivity within the Retina 1113 102 Diagnosis of Autoimmune Blistering Diseases 1120 Section O Cancer 1133 Volume Editor: Maurice R. G. O’gorman Section Editor: Brent L. Wood 103 Introduction 1135 104 Immunoassay- Based Tumor Marker Measurement: Assays, Applications, Algorithms, and Additional Approaches 1136 105 Immune Monitoring during Cancer Immunotherapy 1144 106 Immunophenotyping by Flow Cytometry of Acute Lymphoblastic Leukemia Lymphoma 1168 107 Acute Myeloid Leukemia: Diagnosis and Measurable Residual Disease Detection by Flow Cytometry 1180 108 Chronic Lymphocytic Leukemia: Diagnosis and Minimal Residual Disease Detection 1193 109 Evaluation of Plasma Cell Neoplasms 1206 Section P Transplantation Immunology 1225 Volume Editor: John L. Schmitz Section Editor: Gerald P. Morris 110 Introduction: Immunogenetics, Histocompatibility Testing, and Transplantation 1227 111 Human Leukocyte Antigen Biology and Genetics 1229 112 Human Leukocyte Antigen Genotyping for Allogeneic Transplantations 1240 113 Human Leukocyte Antigen Antibodies 1262 114 Non- HLA Antibodies 1280 115 ABO Blood Groups and Transplantation 1291 116 Cellular Immune Responses in Transplantation 1301 117 Molecular Characterization of Injury and Rejection in Solid Organ Transplant 1312 118 Killer Cell Immunoglobulin- Like Receptors (KIR) in Clinical Transplantation 1323 119 Chimerism Monitoring 1335 Index 1341
Eric T. Weimer
Sarah L. Anzick and Amy P. Hsu
Paul A. Wadsworth, Chandler C. Ho, and Bing M. Zhang
David F. Keren
David M. Manthei and David F. Keren
Caitlin O. Schein and William G. Finn
Peter D. Gorevic, Dennis Galanakis, and Jeffrey S. Warren
David F. Keren and Lee Schroeder
Ashley Frazer- Abel
Diana Pauly and Michael Kirschfink
Amanda K. Slagle, Jill J. Hauer, and Richard J. H. Smith
Bradley Dixon, Shrey Purohit, and Ashley Frazer- Abel
Paul K. Wallace
Ankit Saxena, Angelique Biancotto, Pradeep Dagur, And John Philip Mccoy, Jr
Ahmad Al- Attar, Stephen J. Richards, Iuri Marinov, Andrea Illingworth, And D. Robert Sutherland
Ahmad Al- Attar and D. Robert Sutherland
Bruce Greig
Caleb Cornaby and Maurice R. G. O’gorman
Teri Oldaker and Antony Bakke
Holden T. Maecker
Miranda B. Carper and Thomas N. Denny
Cassandra Porth, Ambrosia Garcia, Raul Louzao, Krystle Garth, Emilie Jalbert, and Adriana Weinberg
David C. Lafon and Moon H. Nahm
Mark Berrong, Danielle Nettere, and Guido Ferrari
Alma- Martina Cepika and Rosa Bacchetta
Heinrich Schlums, Tessa M. Campbell, and Yenan T. Bryceson
Debra Long Priel and Douglas B. Kuhns
Sofia Zavala and Jason E. Stout
Danielle Nettere and Guido Ferrari
John J. Hooks and Timothy B. Niewold
Julio C. Valencia, Ngoc Thuy T. Nguyen, And Howard A. Young
Andrra Nimoni and Timothy B. Niewold
Serena J. Lee, Eszter Toth, Lindsey B. Rosen, Sarah K. Browne, and Steven M. Holland
Thomas S. Alexander
Terri Lebo, Patricia A. Merkel, and Vijaya Knight
Paul R. Johnson
Christine M. Litwin
Christine M. Litwin, Sheldon E. Litwin, And Harry R. Hill
Tyson Chiaro and John L. Schmitz
Guiqing Wang and Maria E. Aguero- Rosenfeld
Christine M. Litwin
Ken B. Waites, Mary B. Brown, and Jerry W. Simecka
Rosemary C. She and Valentina Zanfagnin
Lucas S. Blanton and David H. Walker
Christine M. Litwin, Burt Anderson, Renee Tsolis, and Amy Rasley
Elitza S. Theel
Marc R. Couturier and Elitza S. Theel
Mark D. Lindsley
Jennifer Dien Bard
Susan Realegeno
Henry H. Balfour, Jr., Kristin A. Hogquist, And Priya S. Verghese
Shangxin (shaun) Yang
Rebecca Yee and Jose A. Lucar
Patricia Slev
Bijal A. Parikh and Allison R. Eberly
Ryan F. Relich and Elitza S. Theel
Kileen L. Shier
Mimi R. Precit and Rachael M. Liesman
William Marciel De Souza and Luiz Tadeu Moraes Figueiredo
D. Craig Hooper
Laura Gillim and Ajay Grover
S. Michele Owen
Amanda E. Calvert and Holly R. Hughes
Roshini S. Abraham
Lisa Kobrynski and Mirjam Van Der Burg
Tamar S. Rubin, Kelli W. Williams, and Monica G. Lawrence
Sara Barmettler, Daniel Digiacomo, and Jocelyn R. Farmer
Shanmuganathan Chandrakasan, Gulbu Uzel, Carrie L. Lucas, And Andrew L. Snow
Jenna R. E. Bergerson, Alexandra F. Freeman, Emily M. Mace, And Keith A. Sacco
Xiao Peng, Oskar Schnappauf, Adriana A. De Jesus, and Ivona Aksentijevich
Ann M. Moyer, Meera Sridharan, and Maria Alice Vieira Willrich
Abhishek A. Mangaonkar, Alejandro Ferrer, Asma Ali, Mira Kohorst, and Mrinal M. Patnaik
Elena W. Y. Hsieh, Cullen M. Dutmer, and Vijaya Knight
Robert G. Hamilton
Michael Brad Strader, Robert Zagorski, Ronald L. Rabin, and Jay E. Slater
Robert G. Hamilton
John T. Schroeder
Westley H. Reeves
Angela Ceribelli, Antonio Tonutti, Luis E. C. Andrade, and Edward K. L. Chan
Westley H. Reeves, Shuhong Han, and Haoyang Zhuang
Minoru Satoh, Angela Ceribelli, Tomoko Hasegawa, Shin Tanaka, Michito Hirakata, and Edward K. L. Chan
Yasuhito Hamaguchi and Masataka Kuwana
Johan Rönnelid, Alf Kastbom, and Carl Turesson
Pier Luigi Meroni, Caterina Bodio, Claudia Grossi, and Maria Orietta Borghi
Jan Damoiseaux
R. Hal Scofield and Martha Tsaliki
Kailey E. Brodeur, Liang Chen, And Pui Y. Lee
Gerson Dierley Keppeke, Renan De Almeida Agustinelli, Diana Maria Landoni, Wilson Melo Cruvinel, Edward K. L. Chan, And Luis Eduardo Coelho Andrade
Stanley J. Naides
Amna Kahn, Giuseppe Barbesino, Jonathan Perez, Ziyan Xu, Xiaofan Jia, Fatima Zaman, Liping Yu, and Patrizio Caturegli
Hans Frykman
Raquel Ruiz- García, Laura Naranjo, and Francesc Graus
John A. Goodfellow and Hugh J. Willison
Patrick S. C. Leung, Michael P. Manns, Ross L. Coppel, and M. Eric Gershwin
Jin li
Louma B. Rustam, David E. Elliott, and M. Nedim Ince
Tracey M. Dafonte, Katherine L. Olshan, And Alessio Fasano
Ajay Grover, Andre Valcour, and Stanley J. Naides
Laura Cooling
Qizhao Li, Johan Rebetz, Drew Provan, Leendert L. Porcelijn, Rick Kapur, Suzanne Hofstede- Van Egmond, Elisabeth Semple, Li Guo, Shuqian Xu, and John W. Semple
John J. Hooks, Renee Liu, Lucia Sobrin, And Barbara Detrick
Mareike Becker and Enno Schmidt
Brent L. Wood
Chinwe Madubata and Alex J. Rai
Hesham M. Shehata, Pranay Dogra, and Shomyseh Sanjabi
Karin P. Miller and Alexandra E. Kovach
Xueyan Chen and Brent L. Wood
Min Shi, Dragan Jevremovic, Curtis A. Hanson, and Horatiu Olteanu
Mikhail Roshal, Qi Gao, Shelby Hutcherson, Katie Thoren, Menglei Zhu, And Kazunory Murata
Gerald P. Morris
Bing Melody Zhang and Marcelo Fernandez- Vina
Brian F. Duffy, Mei San Tang, and Chang Liu
Olga A. Timofeeva and Sandra Rosen- Bronson
Carrie L. Butler, Michelle J. Hickey, Rebecca A. Sosa, and Elaine F. Reed
Anne Halpin, Bruce Motyka, and Lori J. West
Clarkson Crane and Gerald P. Morris
Meagan Barner and Zahra Kashi
Raja Rajalingam
Caroline R. Alquist
Subject Areas: Medicine: general issues [MB]
