Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte

A complete guide to intracytoplasmic sperm injection techniques and how to optimize the procedure to ensure success.

Gianpiero D. Palermo (Edited by), Zsolt Peter Nagy (Edited by)

9781108743839, Cambridge University Press

Paperback / softback, published 25 November 2021

172 pages
24.5 x 19 x 1.1 cm, 0.49 kg

For around half of the couples who have trouble conceiving the cause of infertility is sperm-related. Intracytoplasmic sperm injection (ICSI) is the most common and successful treatment for male infertility. Here, the pioneers for the technique, along with authorities in the field, describe the underlying science of ICSI and other micromanipulation techniques. Practical advice for performing the techniques is covered in depth, including sperm selection, laser-assisted ICSI, and the use of piezo in ICSI. Examining the safety of ICSI in animal models as well as the impact of ICSI on the health and well-being of the children conceived through the procedure is discussed. This manual is an essential resource for clinical embryologists and laboratory personnel wishing to refine or develop techniques and improve outcomes.

Foreword Zev Rosenwaks
1. In-vitro fertilization and micromanipulation: The history that changed the treatment of male factor infertility Jacques Cohen
2. Development of ICSI in human assisted reproduction Stephanie Cheung, Alessandra Parrella, Philip Xie, Sherina Lawrence, Mounia Haddad, Derek Keating, Zev Rosenwaks and Gianpiero D. Palermo
3. Current ICSI applications and clinical outcomes Derek Keating, Stephanie Cheung, Philip Xie, June Wang, Zev Rosenwaks and Gianpiero D. Palermo
4. Rescue ICSI of IVF failed-fertilized oocytes Carly Barber, Vivian Nguyen, Alexandrea Ramsey and Zsolt Peter Nagy
5. High-magnification sperm selection before ICSI Pierre Vanderzwalmen, Barbara Wirleitner, Guy Cassuto, Romain Imbert and Maximillian Murtinger
6. Laser-assisted ICSI Roberta Maggiulli, Simona Alfano, Erminia Alviggi, Gemma Fabozzi, Danilo Cimadomo, Filippo Maria Ubaldi and Laura Rienzi
7. Piezo: The add-on to standardize ICSI procedure Tetsunori Mukaida, Kenichiro Hiraoka, Hideaki Watanabe, Kiyotaka Kawai and Csaba Pribenszky
8. Artificial oocyte activation after ICSI Thomas Ebner
9. Health of children born after Intracytoplasmic Sperm Injections (ICSI) Shahad Sabti and Alastair G. Sutcliffe
10. Examining the safety of Intracytoplasmic Sperm Injection (ICSI) using animal models Laura Hewitson
11. Cellular and molecular events after ICSI in clinically relevant animal models Lauren Hamilton, Richard J. Oko and Peter Sutovsky
12. Micromanipulation, microinjection microscopes and systems for ICSI Steven D. Fleming
13. Automation techniques and systems for ICSI Changsheng Dai, Zhuoran Zhang, Guanqiao Shan and Yu Sun
14. Germline nuclear transfer technology to overcome mitochondrial diseases and female infertility Mao-Xing Tang, Annekatrien Boel, Paul Couke and Björn Heindryckx
15. Nuclear transfer technology and its use in reproductive medicine Pasqualino Loi, Marta Czernik, Luca Palazzese, Pier Augusto Scapolo, Helena Fulka and Josef Fulka Jr
16. The prospects of infertility treatment using 'artificial' eggs Katsuhiko Hayashi
Index.

Subject Areas: Pathology [MMF], Gynaecology & obstetrics [MJT], Embryology [MFKC3], Infertility & fertilization [MFKC1], Medical laboratory testing & techniques [MBGL]