Freshly Printed - allow 10 days lead
Couldn't load pickup availability
Liquid-Liquid Phase Coexistence and Membraneless Organelles
A new volume in the Methods in Enzymology series that focuses on enzyme engineering and evolution, including directed evolution
Christine D. Keating (Volume editor)
9780128211595, Elsevier Science
Hardback, published 15 January 2021
406 pages
22.9 x 15.2 x 2.7 cm, 0.76 kg
Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.
1. Using a sequence-specific coarse-grained model for studying protein liquid–liquid phase separation Roshan M. Regy, Wenwei Zheng and Jeetain Mittal 2. Reconstituting bacterial cell division assemblies in crowded, phase-separated media Begoña Monterroso, Miguel Ángel Robles-Ramos, Silvia Zorrilla and Germán Rivas 3. Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform Alexander F. Mason, Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal and Jan C.M. van Hest 4. Cell-free reconstitution of multi-condensate assemblies Andrea Putnam and Geraldine Seydoux 5. Practical considerations for generation of multi-compartment complex coacervates Gregory A. Mountain and Christine D. Keating 6. Methods for characterizing the material properties of biomolecular condensates Ibraheem Alshareedah, Taranpreet Kaur and Priya R. Banerjee 7. Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit Erik W. Martin, Jesse B. Hopkins and Tanja Mittag 8. SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes Amanda B. Marciel, Samanvaya Srivastava, Jeffrey M. Ting and Matthew V. Tirrell 9. Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates Vivek M. Prabhu, Samim Ali, Markus Bleuel, Yimin Mao and Yuanchi Ma 10. Incorporation of proteins into complex coacervates Whitney C. Blocher McTigue and Sarah L. Perry 11. Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation Raghav R. Poudyal, McCauley O. Meyer and Philip C. Bevilacqua 12. Reversible photocontrol of DNA coacervation Suzanne Lafon and Nicolas Martin 13. Enzymatic control over coacervation Karina K. Nakashima, Alain A.M. André and Evan Spruijt
