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Linkers in Biomacromolecules

New volume in the Methods in Enzymology series that focuses on linkers in biomacromolecules

Maarten Merkx (Volume editor)

9780128208182

Hardback, published 21 January 2021

318 pages
22.9 x 15.2 x 2.4 cm, 0.62 kg

Approx.302 pages

Preface Maarten Merkx

1.Understanding and applications of Ser/Gly linkers in protein engineering Eric Ceballos-Alcantarilla and Maarten Merkx

2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineering Ziliang Huang, Chong Zhang and Xin-Hui Xing

3.Engineering crowding sensitivity into protein linkers Theodoros Pittas, Weiyan Zuo and Arnold J. Boersma

4.protaTETHER: A method for the incorporation of linkers in biomacromolecules Anna I. Wurz, Collin T. O'Bryant and Robert M. Hughes

5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase Alejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije

6.Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator? app Magnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes

7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactions Tejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan

8.Design of helical linkers for fusion proteins and protein-based nanostructures Ryoichi Arai

9.Synthetic protein switches: Combinatorial linker engineering with iFLinkC Alexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein

10.Enzyme-DNA chimeras: Construction, allostery, applications Chiao-Yu Tseng, Yong Wang and Giovanni Zocchi

11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry Ri Wang and Fei Sun

Subject Areas: Molecular biology [PSD], Biochemistry [PSB]

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