Light and Electron Microscopy

This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists.

Elizabeth M. Slayter (Author), Henry S. Slayter (Author)

9780521327145, Cambridge University Press

Hardback, published 27 November 1992

332 pages, 144 b/w illus. 11 tables
23.5 x 15.8 x 2.4 cm, 0.662 kg

' … excellent book breaks new ground … rewarding … excellent references (and) good value for money …' Endeavour

The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.

Preface
List of common abbreviations used
1. Introduction
2. Light and electrons
3. Wave interactions
4. Interference effects and diffraction patterns
5. Polarized light
6. Lenses
7. Imaging: microscopy and diffraction
8. Contrast
9. Resolution
10. The light microscope
11. Imaging of phase objects
12. Polarizing microscopy
13. Prospects of biological X-ray microscopy
14. The conventional transmission electron microscope
15. Scanning microscopes
16. Aspects of practical electron microscopy
17. The quest for ultimate EM resolution
18. Innovations in microscope development
19. Photography
Appendix: image location
Author index
Subject index.

Subject Areas: Microscopy [PDND]