Laboratory Methods in Enzymology: Protein Part D
Laboratory Methods in Enzymology

A collection of core protocols concentrating on protein, carefully written and edited by experts

Jon Lorsch (Volume editor)

9780128002797

Hardback, published 9 June 2015

160 pages
22.9 x 15.1 x 1.8 cm, 0.39 kg

Approx.148 pages

1. Purification of His-tagged Proteins
Anne Spriestersbach, Jan Kubicek, Frank Schäfer, Helena Block and Barbara Maertens
2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag
Krisna C. Duong-Ly and Sandra B. Gabelli
3. Immunoaffinity Purification of Proteins
Jennifer M. Kavran and Daniel J. Leahy
4. Affinity Purification of Protein Complexes Using TAP Tags
Erica Gerace and Danesh Moazed
5. Strep-tagged Protein Purification
Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
6. Proteolytic Affinity Tag Cleavage
Helena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer
7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
Erica Gerace and Danesh Moazed
8. Protein Affinity Purification using Intein/Chitin Binding Protein Tags
Sarah F. Mitchell and Jon R. Lorsch
9. Purification of GST-tagged Proteins
Frank Schäfer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek

Subject Areas: Cellular biology [cytology PSF], Enzymology [PSBZ], Biochemistry [PSB], Physiology [MFG]