Laboratory Manual of Microbiology

A K Roy (Author), M.M.Prasad (Author)

9789380235189

Hardback, published 15 January 2010

210 pages
25 x 15 x 1.5 cm, 0.41 kg

The current publication comprises 15 chapters, including techniques for isolating microorganisms from various sources and assessing their growth, as well as analyses of air, soil, and water microbes. Designed to assist graduate and postgraduate students in microbiology, biochemistry, and biotechnology, this text offers practical instruction on procedures outlined in their course syllabi. To facilitate comprehension, the authors have provided flowcharts and diagrams alongside the instructions. They anticipate that this resource will be beneficial in helping students perform the laboratory assignments required in their coursework.

• 1. Organization of microbiology laboratory.
• 2. Preparation of media-liquid and solid for growth of microorganisms.
• 3. Isolation techniques and maintenance of microorganisms.
• 4. Isolation of pure cultures from soil, air, water and seed surface.
• 5. Staining and microscopic examination of microbes.
• 6. Micrometry and measurement of spores.
• 7. Microbial growth.
• 8. Study of mutations by Ames test.
• 9. Assay of antibiotics, antibiotics sensitivity of microbes and demonstration of antibiotic resistance.
• 10. Bacterial transformation.
• 11. Biochemical characterization of enteric bacteria (IMVIC test).
• 12. Isolation and one step growth curve of coliphage.
• 13. Testing of water quality (bacteriological examination of water).
• 14. Culture from body fluids (urine, stool, blood).
• 15. Mixed acid and alcoholic fermentation.