Daniel L. Minor Jr. and Henry M. Colecraft

Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.

1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern

2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless

3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung

4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura

5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx

Methods to control and characterize ion channel function

6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft

7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny

8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein

9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl

10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU

11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS

Methods to characterize ion channel function and expression in physiology and disease

12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko

13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea

14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge

15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang

16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.

17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy

Nenad Bursac