Freshly Printed - allow 10 days lead
Couldn't load pickup availability
Introductory Experiments on Biomolecules and their Interactions
Provides an introduction to basic biochemistry and analysis of the macromolecules required for cell function, infused with original experiments derived from the author’s industry experience
Robert K. Delong (Author), Qiongqiong Zhou (Author)
9780128009697
Paperback / softback, published 22 May 2015
126 pages, 20 illustrations
22.9 x 15.1 x 1 cm, 0.25 kg
Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class.
Foreword and IntroductionLaboratory safety policies, notebook, lab report formatting & other expectationsExperiment 1: An introduction to basic math and operations in the biomolecular laboratory Experiment 2: Buffer preparation and pH Experiment 3: Amino acid properties and an introduction to chromatography Experiment 4: Rapid purification, gel electrophoresis and enzyme activity assay of the firefly protein enzyme luciferase Experiment 5: Hexokinase and G6DH catalyzed reactions of glucose measurementExperiment 6: Polymerase chain reaction and DNA sequence analysisExperiment 7: Investigating protein:nucleic acid interactions by electrophoretic mobility shift assay (emsa)Experiment 8: RNA quantification and degradation kineticsExperiment 9: Microscale preparation of liposome under mock biotechnology conditions: testing its protein interaction Experiment 10: Preparation and characterization of a fluorescently tagged liposome-protein-DNA particle as a simple model “artificial cell?
