Introduction to Optical Microscopy

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Jerome Mertz (Author)

9781108428309, Cambridge University Press

Hardback, published 1 August 2019

462 pages, 164 b/w illus. 192 exercises
25.2 x 19.5 x 2.5 cm, 1.18 kg

'This excellent, perspicuous textbook, based on Fourier and statistical optics, details the derivations of the equations used to mathematize the physics of optical microscopy.' Barry R. Masters, Optics & Photonics News

This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.

1. Introduction
2. Monochromatic wave propagation
3. Monochromatic field propagation through lens
4. Intensity propagation
5. 3D imaging
6. Radiometry
7. Intensity fluctuations
8. Detection noise
9. Absorption and scattering
10. Widefield microscopy
11. Interference microscopy
12. Optical coherence tomography
13. Fluorescence
14. Confocal microscopy
15. Structured illumination microscopy
16. Multiphoton microscopy
17. Multiharmonic microscopy
18. Pump-probe microscopy
19. Superresolution
20. Imaging in scattering media
Appendix A. Properties of Fourier transforms
Appendix B. Miscellaneous math
Appendix C. Jones matrix description of polarization.

Subject Areas: Applied optics [TTB], Electronics engineering [TJF], Nanotechnology [TBN], Microscopy [PDND], Biomedical engineering [MQW], Medical imaging [MMP]