Introduction to Electron Microscopy for Biologists

This volume demonstrates how electron microscopy contributes to knowledge about biological structural information.

Terry D. Allen (Volume editor)

9780123743206, Elsevier Science

Hardback, published 22 October 2008

560 pages
23.4 x 19 x 3.2 cm, 1.2 kg

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).

Scanning Electron Microscopy at the sub-cellular level
Electron Probe X-ray microanalysis for the study of cell physiology
Preparation of cells and tissues for Immuno EM
Fixation, Freezing and Embedding for Genetically Altered Tissue
Combined video fluorescence and 3-D electron microscopy
From Live-cell Imaging to Scanning Electron Microscopy (SEM)
Immunolabelling for High Resolution
Immunogoldlabeling of Thawed Cryosections
Close-to-Native Ultrastructural Preservation by High Pressure Freezing
High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
Freeze-fracture Cytochemistry in Cell Biology
The Plasma membrane cytoskeleton
Correlated Light and Electron Microscopy of the Cytoskeleton
Electron microscopy of intermediate filaments
Studying Microtubules by Electron Microscopy
Endoplasmic reticulum and Golgi Complex
Visualization of Dynamins
Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction
Visualisation of Desmosomes in the Electron Microscope
A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)
High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM
Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
Visualization of Nuclear Organisation by Ultrastructural Cytochemistry
Scanning Electron Microscopy of Chromosomes
Infection at the Cellular Level
Electron Microscopy of Viruses and Virus-Cell Interactions.

Subject Areas: Biotechnology [TCB], Cellular biology [cytology PSF], Molecular biology [PSD], Biochemistry [PSB], Biophysics [PHVN], Microscopy [PDND]