Intermediate Filament Cytoskeleton

An essential resource for methods and protocols for studying intermediate filament proteins.

M. Bishr Omary (Volume editor), Pierre A. Coulombe (Volume editor)

9780125641739

Hardback, published 18 December 2004

855 pages, Approx. 100 illustrations
23.4 x 19 x 4.2 cm, 1.74 kg

Intermediate filaments are a large family of proteins that are the cytoskeletal elements involved in a number of skin, liver, neuromuscular, cardiac, eye and hair diseases. Intermediate filament genes are regulated in a tissue-and cell type-specific manner and their polymerized protein products protects the cells and tissue they are part of against a variety of mechanical and nonmechanical stresses. This book provides a comprehensive resource of methodology essentials, describing a variety of essential tools and assays for studying intermediate filaments. The book provides user-friendly advice and protocols covering all aspects of intermediate filaments including protein isolation and structure, protein and gene regulation, relationship to disease and apoptosis, and associated proteins. Both mammalian and non-mammalian systems and animal models are covered, making this book a must-have for any investigator wishing to study IF genes or their protein products.

Subject Areas: Cellular biology [cytology PSF], Proteins [PSBC]